International Journal of Proteomics

Proteomics Sample Preparation, Preservation, and Fractionation


Publishing date
17 Aug 2012
Status
Published
Submission deadline
17 Feb 2012

Lead Editor

1Covaris, Woburn, MA, USA; Hubbard Center for Genome Studies, University of New Hampshire, Durham, NH 03824, USA

2Queen Elizabeth Hospital, Kowloon, Hong Kong

3Laboratory for Innovative Translational Technologies, Harvard Clinical and Translational Science Center, Boston, MA 02115, USA

4Department of Chemistry, University of Missouri, Columbia, MO 65211, USA


Proteomics Sample Preparation, Preservation, and Fractionation

Description

In even the simplest of organisms, the complexity of its proteomic amalgam is compounded by the broad concentration range over which proteins are expressed, frequently spanning over nine orders of magnitude, as well as an undeterminable number of possible posttranslational modifications that can produce multiple isoforms of many proteins. Hence, neither genomics nor transcriptomics can realistically predict the constituency of the proteomic complement. For instance, there are 3778 distinct genes encoding for proteins that comprise the human plasma proteome, of which at least 51% of these genes encode more than one protein isoform. Consider the variable regions of the immunoglobulins, which collectively are expected to contribute nearly 10 million unique protein sequences to the total number of plasma proteins. Moreover, the search for biologically important proteins of low abundance is further impeded by the enormous range of protein concentrations, as exemplified in human plasma where the mass of albumin is nearly 10 billion times greater than that of important cell-signaling proteins like the interleukins. The diversity of proteins, ranging from very soluble proteins in biological fluids to extremely hydrophobic ones that exist either embedded in lipid membranes or as insoluble aggregates, suggests that the total protein constituency of cells may not be isolated without bias towards or against some protein subpopulations. On the other hand, the complexity of proteomes might be decreased by exploiting the bias toward specific protein subpopulations.

We invite investigators to contribute original research articles as well as review articles on the proteomic aspects of sample preparation, preservation, and fractionation. Potential topics include, but are not limited to:

  • Affinity enrichment of low-abundance proteins and the depletion of high-abundance proteins
  • Isolation and quantification of membrane proteins
  • Preservation and quantification of rare posttranslational modifications of proteins
  • Preservation of in vivo phosphorylation states
  • Preparative and analytical scale sample fractionation strategies
  • New sample preparation technologies
  • Meeting the challenges of particularly difficult or labile samples and proteins
  • Deep proteome research
  • Challenges and advances in microbial proteomics sample preparation, fractionation, and analyses

Before submission authors should carefully read over the journal's Author Guidelines, which are located at http://www.hindawi.com/journals/ijpro/guidelines/. Prospective authors should submit an electronic copy of their complete manuscript through the journal Manuscript Tracking System at http://mts.hindawi.com/ according to the following timetable:


Articles

  • Special Issue
  • - Volume 2012
  • - Article ID 701230
  • - Editorial

Proteomics Sample Preparation, Preservation, and Fractionation

Gary B. Smejkal
  • Special Issue
  • - Volume 2012
  • - Article ID 515372
  • - Research Article

Functional Proteomic Profiling of Phosphodiesterases Using SeraFILE Separations Platform

Amita R. Oka | Matthew P. Kuruc | ... | Swapan Roy
  • Special Issue
  • - Volume 2012
  • - Article ID 536963
  • - Research Article

Optimization of an Efficient Protein Extraction Protocol Compatible with Two-Dimensional Electrophoresis and Mass Spectrometry from Recalcitrant Phenolic Rich Roots of Chickpea (Cicer arietinum L.)

Moniya Chatterjee | Sumanti Gupta | ... | Sampa Das
  • Special Issue
  • - Volume 2012
  • - Article ID 290457
  • - Research Article

Miniaturized Mass-Spectrometry-Based Analysis System for Fully Automated Examination of Conditioned Cell Culture Media

Emanuel Weber | Martijn W. H. Pinkse | ... | Peter D. E. M. Verhaert
  • Special Issue
  • - Volume 2012
  • - Article ID 735132
  • - Research Article

An Economical High-Throughput Protocol for Multidimensional Fractionation of Proteins

David John Tooth | Varun Gopala Krishna | Robert Layfield
  • Special Issue
  • - Volume 2012
  • - Article ID 473843
  • - Research Article

Rapid Screening of the Epidermal Growth Factor Receptor Phosphosignaling Pathway via Microplate-Based Dot Blot Assays

Amedeo Cappione | Janet Smith | ... | Timothy Nadler
  • Special Issue
  • - Volume 2012
  • - Article ID 630409
  • - Research Article

Chromatin-Associated Proteins Revealed by SILAC-Proteomic Analysis Exhibit a High Likelihood of Requirement for Growth Fitness under DNA Damage Stress

Han Wang | Pornpimol Tipthara | ... | Jianhua Liu
  • Special Issue
  • - Volume 2012
  • - Article ID 123076
  • - Research Article

A Comprehensive Subcellular Proteomic Survey of Salmonella Grown under Phagosome-Mimicking versus Standard Laboratory Conditions

Roslyn N. Brown | James A. Sanford | ... | Joshua N. Adkins
  • Special Issue
  • - Volume 2012
  • - Article ID 838630
  • - Research Article

Method for Recovery and Immunoaffinity Enrichment of Membrane Proteins Illustrated with Metastatic Ovarian Cancer Tissues

Luke V. Schneider | Varsha Likhte | ... | Gary B. Smejkal
  • Special Issue
  • - Volume 2012
  • - Article ID 397103
  • - Research Article

Plasma Fractionation Enriches Post-Myocardial Infarction Samples Prior to Proteomics Analysis

Lisandra E. de Castro Brás | Kristine Y. DeLeon | ... | Merry L. Lindsey

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