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International Journal of Polymer Science
Volume 2014, Article ID 921489, 6 pages
http://dx.doi.org/10.1155/2014/921489
Research Article

Enhancement of Fluorescent Labeling via a Composited Thin Film

1Department of Electrical and Computer Engineering, Akashi National College of Technology, Akashi 674-8501, Japan
2Graduate School of Science and Technology, Kumamoto University, Kumamoto 860-8555, Japan
3College of Physics and Electronic Information Engineering, Wenzhou University, Wenzhou 325035, China

Received 2 August 2013; Accepted 30 January 2014; Published 3 March 2014

Academic Editor: Nanda Gopal Sahoo

Copyright © 2014 Taikei Suyama et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Fluorescent labeling is the prevailing imaging technique in cell biological research. When statistical investigations on a large number of cells are involved, experimental study is required for both low magnification to get a reliable statistical population and high contrast to achieve accurate diagnosis on the nature of the cells’ perturbation. As microscope objectives of low magnification generally yield low collection efficiency, such studies are limited by the fluorescence signal weakness. To overcome this technological insufficiency, Le Moal et al. proposed a method based on metal-coated substrates that enhanced the fluorescence process and improved collection efficiency in fluorescence microscope observation and that could be directly used with a common microscope setup. In this paper, we use an Ag-Si3N4-Ag multilayer film coated on the substrate and numerically analyse the optical behavior of a fluorophore which was placed above the composited film coated on the substrate. The results shows that by using an Ag-Si3N4-Ag composited film the fluorescence imaging can be enhanced remarkably.