Enzymatic Digestion and Mass Spectroscopies of N-Linked Glycans in Lacquer Stellacyanin from Rhus vernicifera

Figure 1

Stellacyanin isolated from lacquer sap: (a) SDS-PAGE profile, (b) MALDI TOF MS profile, and (c) amino acid sequence of lacquer stellacyanin. N-glycosylation sites at Asn28, Asn60, and Asn102 are underlined. ▼ and |: trypsin and chymotrypsin cleavage sites, respectively, and B: unidentified amino acid residue, N (asparagine) or D (aspartic acid).

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