Enzymatic Digestion and Mass Spectroscopies of N-Linked Glycans in Lacquer Stellacyanin from Rhus vernicifera
Table 2
MALDI TOF MS assignment of the N-linked glycans from lacquer stellacyanin.
Peak number
Observed
Theoretical
N-linked glycanc
PNGase A
PNGase F
N-glycan
aoWR-glycan
1
2642.08
2641.60
2192.27
2640.48
(HexNAc)4 (Hex)5 (Fuc)3 Xyl
2
2495.98
2495.83
2046.74
2494.95
(HexNAc)4 (Hex)5 (Fuc)2 Xyl
3
ā
2349.64
1900.50
2347.71
(HexNAc)4 (Hex)5 (Fuc) Xyl
4
2130.84
2130.08
1681.60
2129.81
(HexNAc)3 (Hex)4 (Fuc)2 Xyl
5
1984.78
1984.60
1535.55
1983.76
(HexNAc)2 (Hex)4 (Fuc) Xyl
6
1838.68
1838.63
1389.49
1837.70
(HexNAc)3 (Hex)4 Xyl
7
1619.5
ā
1170.41
1618.62
(HexNAc)2 (Hex)3 (Fuc) Xyl
MALDI TOF MS signal digested with PNGase A and PNGase F.
bTheoretical molecular weight of N-linked glycan.
cHexNAc: N-acetylglucosamine, Hex: mannose or glucose, Fuc: fucose, and Xyl: xylose.