Optimization of Process Parameters for <i>ε</i>-Polylysine Production by Response Surface Methods

Zhu, Maxiaoqi; Zhang, Zhicai; Liu, Yiqiuyi; Wang, Feng; Xia, Lili; Xia, Jianwei; Guo, Hongming

doi:https://doi.org/10.1155/2016/3785036

International Journal of Polymer Science

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Abstract Introduction Experimental Results and Discussion Conclusion Abbreviations Acknowledgments Supplementary Materials References Copyright Related Articles

Research Article | Open Access

Volume 2016 | Article ID 3785036 | https://doi.org/10.1155/2016/3785036

Optimization of Process Parameters for ε-Polylysine Production by Response Surface Methods

Maxiaoqi Zhu,¹Zhicai Zhang,¹Yiqiuyi Liu,¹Feng Wang,¹Lili Xia,¹Jianwei Xia,²and Hongming Guo²

Academic Editor: Huining Xiao

Received26 Nov 2015

Revised15 Apr 2016

Accepted09 May 2016

Published28 Jun 2016

Abstract

ε-Polylysine (ε-PL) is a highly safe natural food preservative with a broad antimicrobial spectrum, excellent corrosion resistances, and great commercial potentials. In the present work, we evaluated the ε-PL adsorption performances of HZB-3B and D155 resins and optimized the adsorption and desorption conditions by single-factor test, response surface method, and orthogonal design. The complexes of resin and ε-PL were characterized by SEM and FITR. The results indicated that D155 resin had the best ε-PL adsorption performance and was selected for the separation and purification of ε-PL. The conditions for the static adsorption of ε-PL on D155 resin were optimized as follows: ε-PL solution 40 g/L, pH 8.5, resins 15 g/L, and absorption time 14 h. The adsorption efficiency of ε-PL under the optimal conditions was 96.84%. The ε-PL adsorbed on the D155 resin was easily desorbed with 0.4 mol/L HCl at 30°C in 10 h. The highest desorption efficiency was 97.57% and the overall recovery of ε-PL was 94.49% under the optimal conditions. The excellent ε-PL adsorption and desorption properties of D155 resin including high selectivity and adsorption capacity, easy desorption, and high stability make it a good candidate for the isolation of ε-PL from fermentation broths.

1. Introduction

Natural preservatives are promising food preservatives and have become a major research field worldwide in the recent years. ε-Polylysine (ε-PL) is a polypeptide containing 25 to 30 lysine residues and usually synthesized with Streptomyces [1]. It is one of highly safe natural food preservatives with a broad antimicrobial spectrum, excellent corrosion resistances, and commercial potentials [2–4]. ε-PL can inhibit the growth of Gram-negative bacteria, yeast, and mold in the neutral and acidic environment, especially, for E. coli and Salmonella that cannot be effectively inhibited by other natural preservatives [5–8]. Therefore, it is highly valuable and has a very promising market prospect. Most researches have been focused on breeding high-yield ε-PL strains and optimizing fermentation process to improve ε-PL yields [9, 10]. With the development of fermentation techniques, the extraction and purification of ε-PL in industrial production have become another research focus to lower production cost and improve product quality [11].

Ion exchange and ultra-filtration are two most widely used techniques for ε-PL separation [12]. Ion exchange technique has a great potential in isolation of active components from fermentation broth due to its short process, high recovery, and less pollution and has been extensively used in water treatment [13, 14] and extraction of active components from various media [14–18]. ε-PL is a cationic polymer at pH < pI (9.0) and can be absorbed by cation exchange resin.

In the present work, the ε-PL adsorption performances and conditions of various ion exchange resins were evaluated by single-factor test. The adsorption conditions of ε-PL on the resins were optimized by response surface method. The desorption of ε-PL was optimized with the desorption efficiency by single-factor test and orthogonal array test. Based on the results, an optimum extraction process of ε-PL was proposed.

2. Experimental

2.1. Materials

H⁺ resins HZD-5, HD-2, HZD-3B, D113, and D155 were provided by Shanghai Huazheng Technology Co., Ltd. (Shanghai, China), and were pretreated according to the manufacture’s manual. All other reagents were of analytical grade. ε-PL was a gift from Yiming Biological Co. Ltd. (Jiangshu, China).

2.2. Determination of Adsorption and Desorption Efficiency

Based on the method reported by Itzhaki et al. [18], the adsorption amount, adsorption efficiency, and desorption efficiency were calculated as follows:where is the initial concentration of ε-PL (g/L); is the ε-PL concentration in the adsorption solution (g/L); is the ε-PL concentration in the eluent (g/L); is the volume of the initial ε-PL solution (mL); is the volume of ε-PL solution after adsorption (mL); is the volume of the eluent (mL); is the weight of resin (g); is the absorbed amount of ε-PL (mg/g); is adsorption efficiency; is the desorption efficiency; and is the total recovery.

2.3. Univariate Analysis of ε-PL Adsorption

2.3.1. Effects of pH on ε-PL Adsorption

Five resins were pretreated and, respectively, divided into eight portions of 1 g resin. ε-PL solutions (20 mL 40 g/L) with pH = 2, 3, 4, 5, 6, 7, 8, and 9 were added to the eight aliquots of each resin and allowed a 10 h adsorption at 25°C under stirring at 200 r/min. The concentrations of ε-PL that remained in the solutions were then measured.

2.3.2. Effects of Resin Dosage on ε-PL Adsorption

ε-PL solutions (20 mL, 40 g/L, pH 8.5) were, respectively, added to 0.5 g, 1 g, 1.5 g, and 2 g resins at 25°C and stirred at 200 r/min for 10 h. The concentrations of ε-PL that remained in the solutions were then measured.

2.3.3. Effects of Initial ε-PL Concentration on ε-PL Adsorption

Five portions of 1 g resins were, respectively, mixed with 20 mL 10 g/L, 20 g/L, 30 g/L, 40 g/L, and 50 g/L ε-PL solutions (pH 8.5) at 25°C and stirred at 200 r/min for 10 h. The concentrations of ε-PL that remained in the solutions were then measured.

2.3.4. Effects of Adsorption Time on ε-PL Adsorption

Fifteen portions of 1 g resins were, respectively, added to fifteen 20 mL 40 g/L ε-PL solutions (pH = 8.5) at 25°C and stirred at 200 r/min for 1 h, 2 h, 3 h, 4 h, 5 h, 6 h, 7 h, 8 h, 9 h, 10 h, 11 h, 12 h, 13 h, 14 h, and 15 h, respectively. The concentrations of ε-PL that remained in the solutions were then measured.

2.4. Response Surface Analysis of the Static ε-PL Adsorption

Based on the univariate analysis results, the effects of ε-PL concentration, initial pH, resin dosage, and adsorption time on the ε-PL adsorption of HZD-3B and D155 resins were determined with 29 cycles of a response surface design of four factors and three levels using adsorption efficiency as the index. The regression analysis of the data was conducted with Design-Expert v8.0.6 software. The four factors and three levels of the response surface design of are shown in Table 1.

2.5. Single-Factor Test of Static Desorption of ε-PL

2.5.1. Effects of HCl Concentration

After being rinsed with three volumes of deionized water, ten portions of ε-PL adsorbed resins were, respectively, mixed with 20 mL 0, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, and 1.0 mol/L HCl solutions at 25°C and stirred at 200 r/min for 6 h. The ε-PL concentrations in the HCl solutions were measured.

2.5.2. Effects of Desorption Time

After being rinsed with three volumes of deionized water, ε-PL adsorbed resins were mixed with 20 mL 0.5 mol/L HCl solutions at 25°C and stirred at 200 r/min for 1 h~12 h. The ε-PL concentrations in the HCl solutions were measured.

2.5.3. Effects of Desorption Temperature

After being rinsed with three volumes of deionized water, the ε-PL adsorbed resins were mixed with 20 mL 0.5 mol/L HCl solutions at 15°C, 20°C, 25°C, 30°C, 35°C, and 40°C, respectively, and stirred at 200 r/min for 6 h. The ε-PL concentrations in the HCl solutions were then measured.

2.6. Orthogonal Array Test of the Static Desorption of ε-PL

Based on the single-factor test results, the effects of HCl concentration, desorption time, and desorption temperature on the desorption efficiency of ε-PL from HZD-3B and D155 resins were evaluated by the orthogonal array test with three factors and three levels. The factors and levels for (3³) orthogonal test of the resins are shown in Table 2.

2.7. Data Analysis

All tests were repeated three times and the mean ± SD values were reported. -test was used to determine the statistical significance. was considered as statistical significance.

2.8. Apparatus

Fourier Transform Infrared Spectroscopy (FTIR) was performed on a Thermo-Fisher Nicolet FT-IR spectrometer (USA) in the region of 650–4,000 cm⁻¹ at a resolution of 4 cm⁻¹. The morphologies of the resins with and without ε-PL adsorbed were imaged with a Hitachi S-4800 microscope (Japan).

3. Results and Discussion

3.1. Optimization of Static Adsorption of ε-PL by Single-Factor Test

Ion exchange resins have been widely applied for the high efficiency extraction of bioproducts, especially proteins and polypeptides, from fermentation broth in the form of chromatography [19]. Ion exchange resins can be divided into two types: cation and anion exchange resins. Based on their acidities, cation resins can be classified into strong acid and weak acid cation exchange resins. ε-PL is a weak alkaline homopolymer composed of 25–30 L-lysine residues linked by the peptide bond between carboxyl and ε-amino groups of L-lysine [19–22] and carries a number of positive charges in a specific pH range. Therefore, in the present work, five cation exchange resins were selected to isolate ε-PL from the fermentation broth of Streptomyces sp. Ion exchange chromatography is usually conducted with fixed-bed columns. However, ε-PL aqueous solution has a high viscosity due to the unique structure of ε-PL, which causes long isolation process with fixed-bed columns. Therefore, dynamic adsorption separation is suitable to ε-PL. In the present work, the batch static adsorption and desorption were conducted to evaluate the ε-PL adsorption and desorption properties of the ion exchange resins.

3.1.1. Effects of pH

ε-PL is amphoteric and has an isoelectric point (IP) at ~9.0 [23]. Therefore, pH can affect ε-PL adsorption affinity on resins by alternating the ionization of ε-PL [24]. ε-PL is polyvalent cation at pH lower than 9.0 and is decomposed at pH higher than 9 [25, 26]. Therefore, the adsorption of ε-PL on resins in the pH range of 2~9 was investigated. Figure 1(a) shows the increases in the adsorption efficiencies of ε-PL on all five resins with the increase of pH in the test range, consistent with the conclusion reported by Zhang et al. [27]. The highest ε-PL adsorption efficiencies of five resins were obtained at pH 9 with values of 41.58% for resin D113, 57.48% for resin HZD-5, 69.55% for resin HD-2, 78.60% for D155 resin, and 84.71% for HZD-3B resin. HZD-3B and D155 showed higher ε-PL adsorption capacities and D113 had the lowest ε-PL adsorption capacity.

(a)

(b)

(c)

(d)

3.1.2. Effects of Resin Dosage

The effects of resin dosage on the ε-PL adsorption efficiency are illustrated in Figure 1(b). As can be seen, the ε-PL adsorption efficiencies of all resins increased with the increase of resin dosage. It can be explained that more resins provided more exchangeable sites for ε-PL, resulting in higher adsorption efficiency. The ε-PL adsorption efficiencies of HZD-3B and D155 resins were dramatically increased as the resin dosage increased from 2.5 g/L to 7.5 g/L and slightly increased with the increase of resin dosage from 7.5 g/L to 10 g/L. The ε-PL adsorption efficiencies of other three resins were gradually increased with the increase of the absorbent dosage. The highest adsorption efficiencies were found in 10 g/L D155 and HZD-3B resins with values of 92.63% and 95.23%, respectively.

3.1.3. Effects of Initial ε-PL Concentration

As shown in Figure 1(c), the ε-PL adsorption efficiency of the resins rapidly increased as the initial ε-PL concentration increased from 10 g/L to 20 g/L. It can be explained that large amounts of ε-PL at high concentrations rapidly adsorbed on the resins and consequently saturated the resins. As the ε-PL concentration further increased from 20 g/L to 40 g/L, the adsorption efficiency was gradually increased and the maximum adsorption capacity of the resins was reached. Further increasing initial ε-PL concentration from 40 g/L to 50 g/L led to a slight decline in the ε-PL adsorption efficiency. The ε-PL adsorption efficiencies of all five resins were obtained in 40 g/L ε-PL solution with values of 33.27% for D113 resin, 59.77% for HZD-5 resin, 67.77% for HD-2 resin, 81.7% for D155 resin, and 83.98% for HZD-3B resin. Therefore, initial ε-PL concentration was optimized as 40 g/L. In addition, both HZD-3B and D155 resins showed higher ε-PL adsorption capacities and D113 resin had the lowest ε-PL adsorption capacity under the optimized conditions.

3.1.4. Effects of Adsorption Time

Based on their locations in resins, the adsorption sites are classified into two types: the sites on the surfaces of the resins and the sites in intraresin. The adsorption rates of these two types of sites are different. The adsorbate is rapidly absorbed by the sites on resin surface. In contrast, the adsorbate needs to diffuse into the intraparticle of the resins before being absorbed by the sites in the intraresin [28]. The diffusion to the intraparticle is a slow adsorption process subjected to fast adsorption stage, slow adsorption stage, and equilibrium stage [29–31]. As shown in Figure 1(d) for the ε-PL adsorption efficiency-time curves of the resins (Figure 1(d)), the ε-PL adsorption on all five resins was subjected to three stages with the extension of adsorption time as described above. At the first stage (0–7 h), ε-PL rapidly adsorbed on the resins and the adsorption efficiency linearly increased with the adsorption time. At this stage, the ion exchange adsorption occurred between ε-PL molecules and the ion exchange sites on the surface of resins. The adsorption became slower at the second stage from 7 h to 12 h. During this stage, ε-PL diffused into the interior of resins, adsorbed on the ion exchange sites within the resins and retained in the interior of the resin. Thereafter, during the third stage (after 12 h), the adsorption reached an equilibrium. All ion exchange sites in the resin were saturated and no ion exchange adsorption occurred. The highest adsorption efficiencies on the resins were reached at 12 h with values of 40.46% for D113 resin, 61.94% for HZD-5 resin, 73% for HD-2 resin, 87.67% for D155 resin, and 90.99% for HZD-3B resin. Similarly, HZD-3B and D155 resins showed higher ε-PL adsorption capacities and D113 resin showed lowest adsorption capacity under the testing conditions.

In all, the single-factor tests indicate that all five resins can absorb ε-PL with different adsorption capacities. HZD-3B and D155 resins had higher ε-PL adsorption capacities, followed by HD-2 and HZD-5 resins. D113 resin showed lowest adsorption capacity. Therefore, HZD-3B and D155 resins were selected to further optimize the ε-PL adsorption conditions by response surface method in the following experiments.

3.2. Optimization of the Static Adsorption of ε-PL by Response Surface Method

Based on single-factor tests of the ε-PL adsorption of HZD-3B and D155 resins, pH, resin dosage, initial concentration of ε-PL solution, and adsorption time were selected as independent variables and the adsorption efficiency was used as an dependent variable to further optimize the ε-PL adsorption conditions using Box-Behnken design principle with four factors, three levels, and a total of 29 experiments. Three levels were coded as −1, 0, and +1, respectively [32]. The experimental design and results are shown in Table S1 (see Supplementary Material available online at http://dx.doi.org/10.1155/2016/3785036). The variance analysis of the data was conducted in Design-Expert v8.0.6 software (Table S2).

Regression analysis of the test results was conducted in Design-Expert v8.0.6 software. The fitting quadratic polynomial regression equations are as follows.

For HZD-3B resin,For D155 resin,where is the index value (adsorption efficiency) and , , , and represent pH, resin dosage, initial concentration, and adsorption time, respectively.

The values of the modeling analysis of HZD-3B and D155 resins are 0.9390 and 0.8914, respectively, indicating that the model can fit experimental data well. The high coefficient of determination also suggests a strong correlation between the experimental values and the predicted values in the two models [33]. The regression equation can be used to optimize four parameters in the adsorption process of ε-PL. Both models showed the lack-of-fit , indicating that the regression equation was highly reliable and credible and the experimental error could be negligible. Therefore, the two models can be used to determine the relationship between the independent variables and dependent variables [34]. The quadratic regression equation was able to precisely predict the theoretical value of dependent variable, indicating that the model successfully analyzed and optimized the experimental results.

3.3. Variance Analysis of the ε-PL Adsorption on HZD-3B Resin by Response Surface Method

Both resin dosage and initial ε-PL concentration significantly affected the adsorption of ε-PL on HZD-3B resin (Table ). The effects of resin dosage-pH and initial ε-PL concentration-resin dosage on the ε-PL adsorption of D155 resin reached significant and extremely significant levels, respectively. The response surface plots of the interactions between various factors on the dependent variable (ε-PL adsorption efficiency) were determined and Figure 2 shows the response surface plots of the factors that significantly affected ε-PL adsorption. Steep surfaces indicate more significant effects and flat surfaces represent weaker effects of the factors. Denser contours indicate greater impacts of the factors on the ε-PL adsorption efficiency and vice versa. The contour shape reflects the strength of the interaction between two factors. The circular contours represent weak interactions and less significant effects and oval contours indicate strong interactions and more significant effects.

(a)

(b)

(c)

Based on these results the following can be concluded. (1) The ε-PL adsorption conditions of HZD-3B resin were determined by the response surface method as initial ε-PL concentration 40 g/L, resin dosage 15 g/L, pH 8.65, and adsorption time 12.8 h. The ε-PL adsorption efficiency could reach 99.49% under the optimal conditions. Considering the practical operation process, we proposed the practical optimum adsorption conditions as initial ε-PL concentration 40 g/L, resin dosage 15 g/L, pH 8.5, and adsorption time 13 h. The experimental ε-PL adsorption efficiency was 97.69% under the proposed conditions, consistent with the response surface results. (2) The optimal ε-PL adsorption conditions of D155 resin were determined by the response surface method as initial ε-PL concentration 37.94 g/L, resin dosage 2.85 g/L, pH 8.04, and adsorption time 13.76 h. The ε-PL adsorption efficiency can reach 99.48%. The corrected practical adsorption operation conditions are initial ε-PL concentration 40 g/L, resin dosage 3.0 g, pH 8, and adsorption time 14 h. The experimental ε-PL adsorption efficiency under the optimal operation conditions was 96.84%, consistent with the response surface analysis result. These results indicate the model is reliable.

3.4. Morphologies of D155 and HZD-3B Resins

Figure 3 shows the SEM images of D155 and HZD-3B resins before and after ε-PL adsorbed. Both resins displayed a plicate and porous surface structure. D155 resin possessed large, deep, and sparse pores with a nearly network structure, while HZD-3B resin contained small, shallow, and dense pores (Figures 3(a) and 3(c)). Their porous structure provided more adsorption sites and increased the contact area and diffusion of ε-PL in the resins during the adsorption. The SEM images of the resins after the adsorption indicated that the ε-PL molecules were trapped and adsorbed into the pores and surfaces of resins (Figures 3(b) and 3(d)). The ε-PL layer adsorbed on f D155 resin surface was uniform and thin and that adsorbed on the surface of HZD-3B resin surface was dense and thick.

(a)

(b)

(c)

(d)

3.5. Infrared Spectra Analysis before and after ε-PL Adsorption

ε-PL is a polyamide compound and can be easily identified by its characteristic IR absorption peaks. As can be seen from Figure 4, both resins showed similar characteristic IR spectra after the ε-PL adsorption. The characteristic absorption bands at ~1674.3 cm⁻¹ were attributed to the stretching vibration band of C=O in amide groups (amide absorption band I). The absorption peak at 1570.1 cm⁻¹ was assigned to bending variation of -NH group and stretching vibration of -CN group (amide absorption band II). These two amide absorption bands confirmed the existence of amide groups. The absorption bands at 3270 cm⁻¹ and 3099 cm⁻¹ were attributed to the Fermi resonance generated from the frequency multiplication of the amide absorption band II and the stretching vibration of -NH group, respectively. The peaks at 3431.3 cm⁻¹ and 2944.6 cm⁻¹ were ascribed to the asymmetric stretching of -NH₂ group and stretching of saturate C-H bond, respectively. The peaks at 1461.5 cm⁻¹ and 1436.4 cm⁻¹ were due to CH₂ groups. These results suggest that ε-PL was absorbed on the resin.

(a)

(b)

3.6. Optimization of Static Desorption of ε-PL by Single-Factor Test

The ε-PL adsorbed on the resin can be desorbed with NaOH, NH₄OH, sodium salt, ammonium salt, and acids. However, the pHs of both NaOH and NH₄OH eluents are higher than 9.0, which can cause the degradation of ε-PL [25, 26]. In addition, the high concentrations of salts in the eluents need to be removed to obtain high purity of ε-PL. Acids, especially HCl, for ε-PL desorption, can avoid the decomposition of ε-PL and be removed simply by evaporation under reduced pressure. Therefore, HCl was used to desorb ε-PL from the resins in the present work.

3.6.1. Effects of HCl Concentration

The ε-PL desorption from resins by HCl follows the ion exchange equation:

Therefore, high concentration of HCl is in favor of the ε-PL desorption. However, excessive HCl can increase the production cost and corrode equipment. Hence, the HCl concentration for the ε-PL desorption was first optimized. The ε-PL desorption was conducted in HCl solutions at concentrations ranging from 0.1 to 1.0 mol/L at 25°C for 6 h. As shown in Figures 5(a) and 5(b), the ε-PL desorption efficiency increased with the increase of HCl concentration and peaked at HCl concentration of 0.4 mol/L with values of 83.67% for rHZD-3B resin and 92.88% for D155 resin. Further increasing HCl concentration led to a decline in desorption efficiency. Therefore, 0.4 mol/L HCl was used for ε-PL desorption from the resins.

(a)

(b)

(c)

(d)

(e)

(f)

3.6.2. Effects of Desorption Time

The ε-PL desorption from the resins was conducted in 0.5 mol/L HCl at 25°C for various periods of time. As shown in Figures 5(c) and 5(d), the ε-PL desorption efficiencies of HZD-3B and D155 resins increased from 1 h to 8 h and reached 62.48% and 83.86%, respectively. Further extending desorption time showed no significant effect on the ε-PL desorption efficiency. The desorption time was then optimized as 8 h.

3.6.3. Effects of Desorption Temperature

It has been reported that temperature can significantly influence the equilibrium reaction of ion exchange [35]. The ε-PL desorption was conducted in 0.5 mol/L HCL at various temperatures for 6 h. No significant effects of temperature on the ε-PL desorption were observed (Figures 5(e) and 5(f)). The desorption efficiencies of both HZD-3B and D155 resins increased slightly as the temperature increased from 15°C to 30°C and reached 81.64% and 57.59%, at 30°C, respectively. Further increasing desorption temperature led to a slight decrease in desorption efficiency.

3.7. Optimization of Static Desorption of ε-PL by Orthogonal Array Test

Orthogonal array test has been successfully used in the optimization of bioproduct extractions from fermentation broths and other plant materials [36–38]. In the present study, based on the single-factor analysis results, a (3³) orthogonal array composed of 16 run, 4 factors, and 3 levels was used to optimize desorption conditions of ε-PL from resins. HCl concentration (), desorption time (), and desorption temperature () were used as the processing factors and desorption rate of ε-PL was used as the evaluation indicator (Table 3).

The influences of the processing variables on the indicators were determined using range () analysis (Table 4) and ANOVA (Table S3). The statistical significances of the effects of the factors on the ε-PL desorption from both HZD-3B and D155 resins were in the order of (HCL concentration) > (adsorption temperature) > (adsorption time). -test was then used to determine, at a specific confidence level, whether these factors had significant effects on the ε-PL desorption efficiency of resins. Higher -values indicate more significant effects of the factors. The -test of the variances of the orthogonal array showed that the only factors (HCl concentration) and (desorption temperature) were significant () for ε-PL desorption efficiency (Table S3) and factor (desorption time) was insignificant () for the ε-PL desorption efficiency. The ε-PL desorption conditions for HZD-3B resin were optimized as , for example, 0.4 mol/L HCl, desorption time 8 h and desorption temperature 30°C. The optimal ε-PL desorption conditions of D155 resin were determined as , for example, 0.4 mol/L HCl, desorption time 10 h, and desorption temperature 30°C. Under the optimal conditions, the experimental ε-PL desorption efficiencies of HZD-3B and D155 resins were 87.98% and 97.57%, respectively, indicating that the desorption conditions optimized by the orthogonal array test were reliable.

3.8. ε-PL Adsorption and Desorption Performances of HZD-3B and D155 Resins

Table 5 shows the ε-PL adsorption and desorption performances of HZD-3B and D155 resins. Both HZD-3B and D155 resins showed good adsorption capacities with ε-PL adsorption efficiencies of 97.69% and 96.84%, respectively. Their desorption efficiencies were 87.98% and 97.57%, respectively, under the optimal conditions, which, respectively, resulted in 85.95% and 94.49% ε-PL recoveries. As can be seen, D155 resin is a better option for ε-PL extraction and purification. D155 resin is a macroporous weak acid resin made with a novel polymeric material containing a functional group (-COOH) [39]. The proton can exchange with a cation, and the oxygen atom can coordinate directly with cations, which results in the high sorption capacity, easy regeneration, and convenient operation of the resin [39]. In all, D155 resin is an effective resin for ε-PL sorption of.

4. Conclusion

Among the five test resins, HZD-3B and D155 resins showed better adsorption performances. The ε-PL adsorption conditions of HZD-3B resin were optimized as initial ε-PL concentration 40 g/L, pH 8.5, resin dosage 15 g/L, and adsorption time 13 h. The maximum ε-PL adsorption efficiency was 97.69% under the optimal condition. The ε-PL adsorption conditions of D155 resin were optimized as initial ε-PL concentration 40 g/L, pH of 8.5, resin dosage 15 g/L, and adsorption time 14 h. The maximum ε-PL adsorption efficiency was 96.84% under the optimal condition.

HZD-3B and D155 resins showed the similar variation tendency in ultrastructural surface and infrared spectroscopy before and after the ε-PL adsorption.

The ε-PL desorption conditions of HZD-3B resin were optimized as 0.4 mol/L HCl, desorption time 8 h and desorption temperature 30°C, which resulted in a ε-PL desorption efficiency of 87.98%. Similar desorption conditions were optimized for D155 resin except a longer desorption time of 10 h. The ε-PL desorption efficiency under the optimal conditions was 97.57%. D155 resin gave higher ε-PL recovery, indicating it is a better ion exchange resin for ε-PL extraction.

Abbreviations

ε-PL:

ε-Polylysine.

Competing Interests

The authors declare that they have no competing interests.

Acknowledgments

The authors would like to gratefully and sincerely thank the research grants funded by Jiangsu Yiming Biological Co. Ltd., The Scientific, The National Natural Science Foundation of China (no. 21206177), and Jiangsu Province Social Development Foundation of China (SBE2015730013).

Supplementary Materials

Table S1: In the Supplementary Material Section Box-Behnken design matrix along with the experimental results in the optimization of the conditions of hydrolysis reaction are presented.

Table S2: In the Supplementary Material Section Analysis of variance for the Box-Behnken design in the optimization of the conditions of hydrolysis reaction are presented.

Table S3: In the Supplementary Material Section Variance analysis of regression coefficient in the optimization of the conditions of hydrolysis reaction are presented.

Supplementary Material

References

Y.-T. Ho, S. Ishizaki, and M. Tanaka, “Improving emulsifying activity of ε-polylysine by conjugation with dextran through the Maillard reaction,” Food Chemistry, vol. 68, no. 4, pp. 449–455, 2000.
View at: Publisher Site | Google Scholar
A. H. Chheda and M. R. Vernekar, “Improved production of natural food preservative ε-poly-l-lysine using a novel producer Bacillus cereus,” Food Bioscience, vol. 7, pp. 56–63, 2014.
View at: Publisher Site | Google Scholar
L. Y. Cai, A. L. Cao, F. L. Bai, and J. R. Li, “Effect of ε-polylysine in combination with alginate coating treatment on physicochemical and microbial characteristics of Japanese sea bass (Lateolabrax japonicas) during refrigerated storage,” LWT—Food Science and Technology, vol. 62, no. 2, pp. 1053–1059, 2015.
View at: Publisher Site | Google Scholar
J. Hiraki, T. Ichikawa, S.-I. Ninomiya et al., “Use of ADME studies to confirm the safety of ε-polylysine as a preservative in food,” Regulatory Toxicology and Pharmacology, vol. 37, no. 2, pp. 328–340, 2003.
View at: Publisher Site | Google Scholar
S. Shima, H. Matsuoka, T. Iwamoto, and H. Sakai, “Antimicrobial action of ε-poly-L-lysine,” The Journal of Antibiotics, vol. 37, no. 11, pp. 1449–1455, 1984.
View at: Publisher Site | Google Scholar
S. Jia, C. Xu, Z. Tan et al., “Isolation and characterization of a new epsilon-poly-L-lysine-producing strain TUST-2,” Acta Microbiologica Sinica, vol. 50, no. 2, pp. 191–196, 2010.
View at: Google Scholar
H. Y. Zhu, H. Xu, Q. Wu, and W. W. Chen, “Screening and identification of ε-PL producing strain,” Microbiology China, vol. 32, no. 5, pp. 127–130, 2005.
View at: Google Scholar
S. Duan and W. Zhu, “Isolating of a $ε$ -polylysine-producing strain,” Food and Fermentation Industries, vol. 33, no. 8, pp. 14–17, 2007.
View at: Google Scholar
A. K. Pandey and A. Kumar, “Improved microbial biosynthesis strategies and multifarious applications of the natural biopolymer epsilon-poly-l-lysine,” Process Biochemistry, vol. 49, no. 3, pp. 496–505, 2014.
View at: Publisher Site | Google Scholar
L. M. Zhang, R. Li, F. Dong, A. Y. Tian, Z. J. Li, and Y. J. Dai, “Physical, mechanical and antimicrobial properties of starch films incorporated with ε-poly-l-lysine,” Food Chemistry, vol. 166, pp. 107–114, 2015.
View at: Publisher Site | Google Scholar
S. Jia, C. Xu, and Z. Tan, “Isolation and characterization of a new e-poly-L-lysine -producing strain TUST-2,” Acta Microbiologica Sinica, vol. 38, no. 2, pp. 191–196, 2010.
View at: Google Scholar
S. C. Shukla, A. Singh, A. K. Pandey, and A. Mishra, “Review on production and medical applications of e{open}-polylysine,” Biochemical Engineering Journal, vol. 65, pp. 70–81, 2012.
View at: Publisher Site | Google Scholar
C. Xiong, Y. Li, G. Wang et al., “Selective removal of Hg(II) with polyacrylonitrile-2-amino-1,3,4-thiadiazole chelating resin: batch and column study,” Chemical Engineering Journal, vol. 259, no. 1, pp. 257–265, 2015.
View at: Publisher Site | Google Scholar
C. H. Xiong, Y. Q. Zheng, Y. J. Feng et al., “Preparation of a novel chloromethylated polystyrene-2-amino-1,3,4-thiadiazole chelating resin and its adsorption properties and mechanism for separation and recovery of Pt(IV) from aqueous solutions,” Journal of Materials Chemistry A, vol. 2, no. 15, pp. 5379–5386, 2014.
View at: Publisher Site | Google Scholar
A. A. Abdelgadir, L. Boudesocque-Delaye, I. Thery-Koné, A. Gueiffier, E. M. Ahmed, and C. Enguehard-Gueiffier, “One-step preparative isolation of aristolochic acids by strong ion-exchange centrifugal partition chromatography,” Separation and Purification Technology, vol. 156, no. 17, pp. 444–449, 2015.
View at: Publisher Site | Google Scholar
R. S. Gilbert, G. G. Gonzalez, L. Hawel III, and C. V. Byus, “An ion-exchange chromatography procedure for the isolation and concentration of basic amino acids and polyamines from complex biological samples prior to high-performance liquid chromatography,” Analytical Biochemistry, vol. 199, no. 1, pp. 86–92, 1991.
View at: Publisher Site | Google Scholar
H. Zong, Y. Zhan, X. Wu, A. Ru-han, and F. Q. Feng, “Ion exchange resin separation and purification of $ε$ -poly-lysinefrom the fermentation broth of streptomyces violaceusniger,” Journal of Food Science, vol. 32, no. 1, pp. 138–141, 2011.
View at: Google Scholar
R. F. Itzhaki, “Colorimetric method for estimating polylysine and polyarginine,” Journal of Analytical Biochemistry, vol. 50, no. 2, pp. 569–574, 1972.
View at: Publisher Site | Google Scholar
A. Lyddiatt, “Process chromatography: current constraints and future options for the adsorptive recovery of bioproducts,” Current Opinion in Biotechnology, vol. 13, no. 2, pp. 95–103, 2002.
View at: Publisher Site | Google Scholar
J. Hiraki, M. Hatakeyama, H. Morita, and Y. Izumi, “Improved ξ-poly-L-lysine production of an S-(2-aminoethyl)-L-cysteine resistant mutant of Streptomyces albulus,” Seibutsu-Kogaku Kaishi, vol. 76, no. 12, pp. 487–493, 1998.
View at: Google Scholar
T. Yoshida and T. Nagasawa, “ε-poly-L-lysine: microbial production, biodegradation and application potential,” Applied Microbiology and Biotechnology, vol. 62, no. 1, pp. 21–26, 2003.
View at: Publisher Site | Google Scholar
K. G. Zinoviadou, K. P. Koutsoumanis, and C. G. Biliaderis, “Physical and thermo-mechanical properties of whey protein isolate films containing antimicrobials, and their effect against spoilage flora of fresh beef,” Food Hydrocolloids, vol. 24, no. 1, pp. 49–59, 2010.
View at: Publisher Site | Google Scholar
A. T. Cao and S. R. Xiao, “Structure and application of -polylysine,” China Brewing, vol. 188, no. 11, pp. 11–14, 2008.
View at: Google Scholar
M. Iwata, M. S. Jami, and M. Sato, “Analysis of constant-current electro-osmotic dewatering of various solid-liquid systems by considering the creep deformation,” Separation and Purification Technology, vol. 58, no. 2, pp. 274–281, 2007.
View at: Publisher Site | Google Scholar
P. Kahar, T. Iwata, J. Hiraki, E. Y. Park, and M. Okabe, “Enhancement of ε-polylysine production by Streptomyces albulus strain 410 using pH control,” Journal of Bioscience and Bioengineering, vol. 91, no. 2, pp. 190–194, 2001.
View at: Publisher Site | Google Scholar
H. T. Zhang, Y. Li, J. Ou, and W. Q. Wu, “Resin Screening for the Isolation of $ε$ -polylysine from fermentation broth,” Journal of Chemical Research Applications, vol. 20, no. 1, pp. 108–112, 2008.
View at: Google Scholar
H. T. Zhang, Y. Li, J. Ou, and W. Q. Wu, “Screening of resins available for separating $ε$ -polylysine from ferment broth,” Chemical Research and Application, vol. 20, no. 1, pp. 108–112, 2008.
View at: Google Scholar
I. Ghodbane and O. Hamdaoui, “Removal of mercury(II) from aqueous media using eucalyptus bark: kinetic and equilibrium studies,” Journal of Hazardous Materials, vol. 160, no. 2-3, pp. 301–309, 2008.
View at: Publisher Site | Google Scholar
W. Luo, H. Chen, L. M. Fan et al., “Adsorption behavior of L-tryptophan on ion exchange resin,” Korean Journal of Chemical Engineering, vol. 28, no. 5, pp. 1272–1279, 2011.
View at: Publisher Site | Google Scholar
A. A. Hekmatzadeh, A. Karimi-Jashni, N. Talebbeydokhti, and B. Kløve, “Adsorption kinetics of nitrate ions on ion exchange resin,” Desalination, vol. 326, no. 10, pp. 125–134, 2013.
View at: Publisher Site | Google Scholar
K. H. Chu and M. A. Hashim, “Protein adsorption on ion exchange resin: estimation of equilibrium isotherm parameters from batch kinetic data,” Biotechnology and Bioprocess Engineering, vol. 11, no. 1, pp. 61–66, 2006.
View at: Publisher Site | Google Scholar
M. Z. Alam, M. F. Mansor, and K. C. A. Jalal, “Optimization of decolorization of methylene blue by lignin peroxidase enzyme produced from sewage sludge with Phanerocheate chrysosporium,” Journal of Hazardous Materials, vol. 162, no. 2-3, pp. 708–715, 2009.
View at: Publisher Site | Google Scholar
P. Gupta and P. Parkhey, “A two-step process for efficient enzymatic saccharification of rice straw,” Bioresource Technology, vol. 173, pp. 207–215, 2015.
View at: Publisher Site | Google Scholar
S. Tahmouzi, “Optimization of polysaccharides from Zagros oak leaf using RSM: antioxidant and antimicrobial activities,” Carbohydrate Polymers, vol. 106, no. 1, pp. 238–246, 2014.
View at: Publisher Site | Google Scholar
V. A. Ivanov, V. I. Gorshkov, V. D. Timofeevskaja, and N. V. Drozdova, “Influence of temperature on ion-exchange equilibrium accompanied by complex formation in resins,” Reactive and Functional Polymers, vol. 38, no. 2-3, pp. 205–218, 1998.
View at: Publisher Site | Google Scholar
Z. C. Zhang, Y. Li, and K. C. Zhang, “Application of statistical analysis for the optimization of mycelia and polysaccharide production by Tremella aurantialba,” Food Technology and Biotechnology, vol. 45, no. 1, pp. 45–50, 2007.
View at: Google Scholar
A. A. Patil, B. S. Sachin, P. S. Wakte, and D. B. Shinde, “Optimization of supercritical fluid extraction and HPLC identification of wedelolactone from Wedelia calendulacea by orthogonal array design,” Journal of Advanced Research, vol. 5, no. 6, pp. 629–635, 2014.
View at: Publisher Site | Google Scholar
T. Wu, J. Yan, R. Liu, M. F. Marcone, H. A. Aisa, and R. Tsao, “Optimization of microwave-assisted extraction of phenolics from potato and its downstream waste using orthogonal array design,” Food Chemistry, vol. 133, no. 4, pp. 1292–1298, 2012.
View at: Publisher Site | Google Scholar
C. H. Xiong, “Study on sorption of D155 resin for gadolinium,” Journal of Rare Earths, vol. 26, no. 2, pp. 258–263, 2008.
View at: Publisher Site | Google Scholar

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Copyright © 2016 Maxiaoqi Zhu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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