Incorporation of Amphipathic Diblock Copolymer in Lipid Bilayer for Improving pH Responsiveness

Table 2

Compositions and properties of the liposomes (pH 7.4).

Liposome	Composition of liposome	Copolymer percentage	Diameter (nm)	PDI	Zeta-potential (mV)
Lp1	HSPC	0	139.3 ± 4.0	0.151	10.3 ± 0.8
Lp2	HSPC : polymer; (mPEG8-b-PDPA10)	5%	173.2 ± 8.0	0.229	13.6 ± 1.4
Lp3	HSPC : polymer; (mPEG8-b-PDPA15)	5%	183.7 ± 8.0	0.246	15.8 ± 1.4
Lp4	HSPC : polymer; (mPEG8-b-PDPA22)	2%	179.9 ± 8.0	0.219	15.7 ± 1.4
Lp5	HSPC : polymer; (mPEG8-b-PDPA22)	5%	198.8 ± 8.0	0.250	16.9 ± 1.4
Lp6	HSPC : polymer; (mPEG8-b-PDPA22)	10%	206.2 ± 8.0	0.334	18.7 ± 1.4
Lp7	HSPC : DOPC (1 : 1)	0	146.0 ± 4.0	0.178	11.6 ± 1.4
Lp8	HSPC : DOPC (1 : 1) : polymer; (mPEG8-b-PDPA10)	5%	163.5 ± 8.0	0.207	17.2 ± 1.4
Lp9	HSPC : DOPC (1 : 1) : polymer; (mPEG8-b-PDPA15)	5%	175.1 ± 8.0	0.210	16.1 ± 1.4
Lp10	HSPC : DOPC (1 : 1) : polymer; (mPEG8-b-PDPA22)	5%	187.3 ± 8.0	0.207	17.2 ± 1.4

Cholesterol was contained in all liposomes and the molar ratio between lipid and cholesterol was 5 : 1.