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International Journal of Polymer Science
Volume 2016 (2016), Article ID 6541718, 12 pages
http://dx.doi.org/10.1155/2016/6541718
Research Article

Production and Characterization of Polyhydroxyalkanoates and Native Microorganisms Synthesized from Fatty Waste

1Área Curricular de Biotecnología, Universidad Nacional de Colombia, Sede Medellín, Medellín, Colombia
2Escuela de Química, Facultad de Ciencias, Universidad Nacional de Colombia, Sede Medellín, Medellín, Colombia
3Departamento de Ciencias Agronómicas, Facultad de Ciencias Agrarias, Universidad Nacional de Colombia, Sede Medellín, Medellín, Colombia

Received 28 August 2015; Accepted 4 January 2016

Academic Editor: Antonio Teixeira

Copyright © 2016 Javier Ricardo Gómez Cardozo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Polyhydroxyalkanoates (PHAs) are biodegradable and biocompatible plastics. They are synthesized by a wide variety of microorganisms (i.e., fungi and bacteria) and some organisms such as plants, which share characteristics with petrochemical-based plastics. The most recent studies focus on finding inexpensive substrates and extraction strategies that allow reducing product costs, thus moving into a widespread market, the market for petroleum-based plastics. In this study, the production of polyhydroxybutyrate (PHB) was evaluated using the native strains, Bacillus megaterium, Bacillus sp., and Lactococcus lactis, and glycerol reagent grade (GRG), residual glycerol (RGSB) byproduct of biodiesel from palm oil, Jatropha oil, castor oil, waste frying oils, and whey as substrates. Different bacteria-substrate systems were evaluated thrice on a laboratory scale under different conditions of temperature, pH, and substrate concentration, employing 50 mL of broth in 250 mL. The bacterial growth was tested in all systems; however, the B. megaterium GRG system generated the highest accumulation of PHA. The previous approach was allowed to propose a statistical design optimization with RGSB (i.e., RGSB, 15 g/L, pH 7.0, and 25°C). This system reached 2.80 g/L of PHB yield and was the optimal condition tested; however, the optimal biomass 5.42 g/L occurs at pH 9.0 and 25°C, with a substrate concentration of 22 g/L.