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International Journal of Polymer Science
Volume 2016, Article ID 6723716, 9 pages
http://dx.doi.org/10.1155/2016/6723716
Research Article

Fabrication of Hyaluronan-Poly(vinylphosphonic acid)-Chitosan Hydrogel for Wound Healing Application

1Department of Biomedical Engineering, International University, Vietnam National University-Ho Chi Minh City (VNU-HCM), Quarter 6, Linh Trung Ward, Thu Duc District, Ho Chi Minh City 70000, Vietnam
2School of Biotechnology, International University, Vietnam National University-Ho Chi Minh City (VNU-HCM), Quarter 6, Linh Trung Ward, Thu Duc District, Ho Chi Minh City 70000, Vietnam
3Institute of Applied Materials Science, Vietnam Academy of Science and Technology, Ho Chi Minh City 70000, Vietnam
4The Center for Molecular Biomedicine, University of Medicine and Pharmacy, Ho Chi Minh City 70000, Vietnam

Received 7 January 2016; Accepted 10 March 2016

Academic Editor: Matthew Green

Copyright © 2016 Dang Hoang Phuc et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A new hydrogel made of hyaluronan, poly(vinylphosphonic acid), and chitosan (HA/PVPA/CS hydrogel) was fabricated and characterized to be used for skin wound healing application. Firstly, the component ratio of hydrogel was studied to optimize the reaction effectiveness. Next, its microstructure was observed by light microscope. The chemical interaction in hydrogel was evaluated by nuclear magnetic resonance spectroscopy and Fourier transform-infrared spectroscopy. Then, a study on its degradation rate was performed. After that, antibacterial activity of the hydrogel was examined by agar diffusion method. Finally, in vivo study was performed to evaluate hydrogel’s biocompatibility. The results showed that the optimized hydrogel had a three-dimensional highly porous structure with the pore size ranging from about 25 µm to less than 125 µm. Besides, with a degradation time of two weeks, it could give enough time for the formation of extracellular matrix framework during remodeling stages. Furthermore, the antibacterial test showed that hydrogel has antimicrobial activity against E. coli. Finally, in vivo study indicated that the hydrogel was not rejected by the immune system and could enhance wound healing process. Overall, HA/PVPA/CS hydrogel was successfully fabricated and results implied its potential for wound healing applications.