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International Journal of Polymer Science
Volume 2018, Article ID 5049728, 7 pages
Research Article

Evaluation of the Morphology and Biocompatibility of Natural Silk Fibers/Agar Blend Scaffolds for Tissue Regeneration

1Tissue Engineering and Regenerative Medicine Group, Department of Biomedical Engineering, International University, Vietnam National University, Ho Chi Minh City (VNU-HCMC), Ho Chi Minh City 700000, Vietnam
2The Center for Molecular Biomedicine, University of Medicine and Pharmacy, Ho Chi Minh City 700000, Vietnam
3Research Laboratories of Saigon Hi-Tech Park, Ho Chi Minh City 700000, Vietnam
4Institute of Applied Materials Science, Vietnam Academy of Science and Technology, 01 Mac Dinh Chi, District 1, Ho Chi Minh City, Vietnam
5Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Hanoi, Vietnam
6Faculty of Applied Sciences, Ton Duc Thang University, Ho Chi Minh City 700000, Vietnam

Correspondence should be addressed to Nguyen Thi Hiep; moc.liamg@1891peihtn

Received 26 August 2017; Accepted 20 November 2017; Published 18 January 2018

Academic Editor: Kajal Ghosal

Copyright © 2018 Luong Thu-Hien et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This study was aimed to develop a tissue engineering scaffold by incorporation of Bombyx mori silk fiber (BMSF) and agar. This promised the improvement in enhancing their advantageous properties as well as limiting their defects without occurring chemical reactions or crosslink formation. The morphology and chemical structure of scaffolds were observed using scanning electron microscope (SEM) observation and Fourier transform infrared (FT-IR) spectra. The SEM results show that scaffolds containing BMSF have microporous structures, which are suitable for cell adhesion. Agar scaffolds, by contrast, had much more flat morphology. FT-IR spectra confirm that no modifications to BMSF happened in scaffolds, which indicates that there was no chemical reaction or crosslink formation between silk and agar in this process. Furthermore, the biocompatibility of scaffolds was performed in the mouse’s subcutaneous part of the dorsal region for 15 days, followed by Haematoxylin and Eosin (H&E) staining. H&E staining results demonstrate that scaffolds had good biocompatibility and there was no sign of the body rejection in all of samples. The results from animal study show that SA scaffolds have the most stable structure for cell adhesion compared with those single materials.