A Process Engineering Approach to Improve Production of P(3HB) by Cupriavidus necator from Used Cooking Oil

Table 1

Parameters obtained for cultivation of C. necator DSM 428 under fed-batch mode using an exponential feeding profile (experiment A) and the DO-stat mode (experiment B) to feed UCO to the culture. μ_max: maximum specific cell growth rate; X: active biomass; CDM: cell dry mass; P(3HB) content: polymer content in the biomass; P(3HB): polymer concentration; r_p: volumetric productivity; Y_x/s: growth yield; Y_p/s: storage yield.

Exp.	Feeding strategy	(h^−1)	(g L^−1)	CDM (g L^−1)	P(3HB) content (wt%)	P(3HB) (g L^−1)	(g L^−1 day^−1)	(gx gs^−1)	(gp gs^−1)
A	Exponential profile	0.14 ± 0.02	3.4 ± 0.4	21.3 ± 0.9	84.0 ± 4.5	17.9 ± 0.9	4.5 ± 0.2	0.11 ± 0.01	0.65 ± 0.03
B	DO-stat	0.21 ± 0.01	7.8 ± 0.6	27.2 ± 0.5	77.0 ± 5.7	19.8 ± 1.8	12.6 ± 0.8	0.21 ± 0.02	0.52 ± 0.07