Basal Activation of Type I Interferons (Alpha2 and Beta) and OAS Genes: Insights into Differential Expression Profiles of Interferon System Components in Systemic Sclerosis
Figure 1
Type I and type III IFN mRNA basal levels in PBMCs from healthy donors and SSc patients. PBMCs from healthy donors and SSc patients were purified, and total RNA extraction was performed. The RNA obtained was used as template in reverse transcription reactions, and the resulting cDNA was used in real-time PCRs to measure IFN alpha (a), IFN beta (b), IFN lambda1 (c), and IFN lambda 2/3 (d).
