Immune Mediators in Osteoarthritis: Infrapatellar Fat Pad-Infiltrating CD8+ T Cells Are Increased in Osteoarthritic Patients with Higher Clinical Radiographic Grading

Figure 3

Activation phenotype of T cells isolated from peripheral blood, synovial tissue, and infrapatellar fat pad of knee osteoarthritis patients. Mononuclear cells were isolated from peripheral blood, synovial tissue, and infrapatellar far pad labeled for CD69 surface expression. (a) Graphs showing total T cell and T cell subset (CD4+ and CD8+) frequencies in peripheral blood (PBMC) (), synovial tissue (), and infrapatellar fat pad (). Mean values are shown with error bars. (b) Bar graphs showing the mean frequency of CD69+ total T cells and CD69+ T cell subsets (CD4+ and CD8+) stratified based on the Ahlback classification for radiographic grading. Bars represent mean values with error bars. (c) Comparison of frequency of CD4+CD69+ and CD8+CD69+ T cells in peripheral blood, synovial tissue, and infrapatellar fat pad. Shown are mean values. was considered significant. (; ; .)

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