Protein Interactions Investigated by the Raman Spectroscopy for Biosensor Applications

Table 1

Interpretation of the reference Raman spectra of proteins SpA and IgG. The bands characteristics of SpA or IgG, respectively, are indicated in bold letters for characterization of the intensity level: vw: very weak, w: weak, m: medium, s: strong, vs: very strong.

Frequency (cm^−1)	Compound	Assignment	Intensity
647	SpA/IgG	Tyrosine	vw/m
758	SpA/IgG	Tyrosine	vw/m
832	SpA/IgG	Tyrosine	w/m
857	SpA/IgG	Tyrosine	w/m
880–920	SpA/IgG	ρCH2	m/m
933	SpA	δCH3	w
958	SpA/IgG	ρCH3	w/w
1004	SpA/IgG	Phenylalanine	s/vs
1032–1170	SpA/IgG	νC–C or alkyl C–N	w/w
1243	IgG	Amide III	vs
1336	SpA	Amide III	vs
1416	SpA/IgG	Tryptophan	s/vs
1451	SpA/IgG	ρCH2	vs/vs
1552	SpA/IgG	Tryptophan	w/w
1610	SpA/IgG	Phenylalanine	m/m
1659	SpA	Amide I	vs
1673	IgG	Amide I	vs
2860	SpA/IgG	νC–H	m/w
2940	SpA/IgG	νC–H	m/w