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International Journal of Spectroscopy
Volume 2013 (2013), Article ID 502310, 6 pages
http://dx.doi.org/10.1155/2013/502310
Research Article

Development and Validation of First-Order Derivative Spectrophotometry for Simultaneous Determination of Levocetirizine Dihydrochloride and Phenylephrine Hydrochloride in Pharmaceutical Dosage Form

1Department of Pharmaceutical Analysis, Indukaka Ipcowala College of Pharmacy, Beyond GIDC Phase IV, P.O. Box 53, Vithal Udyog Nagar, New Vallabh Vidyanagar 388 121, Gujarat, India
2Department of Pharmaceutics, Parul Institute of Pharmacy and Research, Limda, Ta. Waghodia, Vadodara 391 760, Gujarat, India

Received 28 February 2013; Revised 17 May 2013; Accepted 8 June 2013

Academic Editor: L. J. Ming

Copyright © 2013 Kaminee Parmar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A simple, precise, accurate, and economical spectrophotometric method has been developed for simultaneous estimation of levocetirizine dihydrochloride (LCT) and phenylephrine hydrochloride (PHE) by employing first-order derivative spectrophotometric method. The first-order derivative absorption at 240 nm (zero crossing point of PHE) was used for quantification of LCT and 283.2 nm (zero crossing point of LCT) for quantification of PHE. The linearity was established over the concentration range of 4–24 μg/mL and 8–48 μg/mL for LCT and PHE with correlation coefficients () 0.9964 and 0.9972, respectively. The mean % recoveries were found to be in the range of 99.14%–100.43% for LCT and 98.73%–100.83% for PHE. The proposed method has been validated as per ICH guideline and successfully applied for the simultaneous estimation of LCT and PHE in combined tablet dosage form.