Table 1: Percent decrease in fluorescence intensity of fluorophore (1.0 × 10−5 M) at the highest quencher concentration (21 μM) at 25°C. Fluorescence intensity was measured at 345 nm.

3456

BSA21.829.64.29
HSA19.616.034.6
Lyso23.715.211.0
Trp39.1
Charge0000

Decrease at 1.8 × 10−5 M. Quenching reversed at quencher concentrations higher than 1.8 × 10−5 M.