Annular Beam Shaping in Multiphoton Microscopy to Reduce Out-of-Focus Background

Figure 6

Results from the computer simulations estimating the out-of-focus signal () generated for different shapes of the annular beam given as the ratio . The presented simulations were performed using a mathematical approach considering the varying geometrical distribution of the excitation light depending on . Results are presented for two scenarios assuming different probabilities of collection efficiency (): (a) illustrating Gaussian and (c) flat collection efficiency. The respective results for simulated out-of-focus signal are presented in (b) and (d). For both cases, the value of the attenuation coefficient () was set to 150 cm⁻¹.

(a)

(b)

(c)

(d)