Table of Contents
International Journal of Tissue Engineering
Volume 2013 (2013), Article ID 976894, 7 pages
http://dx.doi.org/10.1155/2013/976894
Research Article

Studies on a Novel Bioreactor Design for Chondrocyte Culture

1School of Biochemical Engineering, Indian Institute of Technology, Banaras Hindu University, Varanasi, India
2Department of Orthopedic Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India

Received 8 April 2013; Revised 17 June 2013; Accepted 7 August 2013

Academic Editor: Vassilios I. Sikavitsas

Copyright © 2013 Harshad Patil et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A bioreactor system plays an important role in tissue engineering and enables reproduction and controlled changes in the environmental factor. The bioreactor provides technical means to perform controlled processes in safe and reduced reproducible generation of time. Cartilage cells were grown in vitro by mimicking the in vivo condition. The basic unit of cartilage, that is, chondrocyte, requires sufficient shear, strain, and hydrodynamic pressure for regular growth as it is nonvascular tissue. An attempt has been made to design a novel airlift reactor for chondrocyte culture, and the reactor has been evaluated for its performance. The design includes internal loop wavy riser airlift reactor for chondrocyte culture with 5% CO2 sparging which gives a good yield of chondrocyte after 28 days. The wavy riser provides more surfaces for collision of fluid flow so to create the turbulence. Also, the horizontal semicircular baffles create an angle of 180° which helps in high shear rate. The optimized L/D ratio of the designed airlift reactor (for chondrocyte culture) is 5.67, and it also exhibits good mixing performance.