In Vivo Evaluation of a Decellularized Limbal Graft for Limbal Reconstruction

Figure 1

Experiment was designed to create a focal limbal injury by laser followed by grafting with a decellularized human limbus. (a) Nidek EC-5000 excimer laser system was used to ablate corneal tissue under anesthesia from a rat eye at the limbal area (junction of cornea and sclera). Arrows pointing to the area of ablation. Inset shows ablated area stained with fluorescein. (b) Decellularized human cornea. (c) Decellularized human cornea grafted on the ablated rat eye. Arrows pointing to the graft.