A Diet Enriched in Docosahexanoic Acid Exacerbates Brain Parenchymal Extravasation of Apo B Lipoproteins Induced by Chronic Ingestion of Saturated Fats
Figure 2
Three-dimensional (3D) quantitative analysis of apo B leakage in C57BL/6J mouse brain. Pixel intensity of apo B lipoproteins surrounding the cerebrovasculature was quantitated in 3D images from mice maintained on saturated-fat diet for 5 months (SFA 5 m) and SFA-fed mice randomised to LF (+LF 2 m) and DHA (+DHA 2 m) diet for further 2 months. The extent of apo B pixel intensity was measured in the cortex (CTX), brain stem (BS), and hippocampal formation (HPF) and expressed as per unit volume. The bars represent mean intensity and standard error of mean, where considered statistically significant (* Kruskal Wallis t-test). The C57BL/6J mice randomised to DHA diet (+DHA) had elevated apo B intensities in all regions of the brain.
