The Oxidative State of Chylomicron Remnants Influences Their Modulation of Human Monocyte Activation
Generation of reactive oxygen species after coculture of CRLP with primary human blood monocytes. Monocytes were preloaded with dihydrorhodamine 1, 2, 3 before incubation with CRLPs, oxCRLPs, or pCRLPs and measurement of fluorescence (ROS production) at increasing timepoints. (a, b, c) Time course of ROS production (fluorescence) 7.5 μg/mL, 15 μg/mL and 30 μg/mL cholesterol, respectively. (d, e, f) area under curve analysis (Prism) 7.5 μg/mL, 15 μg/mL and 30 μg/mL cholesterol respectively. monocyte isolations. *, **, *** paired t-test.
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