Myosin VI and Associated Proteins Are Expressed in Human Macrophages but Do Not Play a Role in Foam Cell Formation in THP-1 Cells

Figure 8

Effects of LDL and oxLDL on expression of mRNA and protein for Myo6 and related proteins in THP-1 macrophages. THP-1 cells were incubated with nLDL or oxLDL (50 μg protein/mL) or an equal volume of PBS (control) for 8 h or 24 h, and total RNA or protein was isolated. The abundance of transcripts for (a) Myo6, (b) Dab2, (c) AP-2α2, and (d) GIPC1 was determined by qPCR (quantified using a standard curve and normalised using geNorm), and the expression of protein for (e) Myo6 and (f) Dab2 was assessed by immunoblotting using β-actin as the housekeeping gene (Con, control). Band density was quantified using Quantity One software (Biorad), and results for (g) Myo6 and (h) Dab2 are expressed as % control value. Data shown ((a)–(d), g, h) are the mean from 4 separate experiments and error bars show the SEM. Significance limits *𝑃<0.05, **𝑃<0.01, ***𝑃<0.001 versus control; ^#𝑃<0.05, ^##𝑃<0.01 versus nLDL (one-way ANOVA).

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