Inhibition of Angiogenesis In Vitro by Chebulagic Acid: A COX-LOX Dual Inhibitor
Figure 5
Effect of CA on the expression of VE cadherin. HUVECs were maintained in culture in MCDB 131 medium containing 10% FBS supplemented with 25 μM CA for 48 hours. (a) The level of VE cadherin in the cell layer was estimated using anti-VE cadherin antibody. Values given are the average of duplicate analysis of 5 experiments ± SEM (). (b) Western blot to analyze the production of VE cadherin by control cells (C) and chebulagic acid treated cells (CA) at 48 hours. The intensity of the immunoblotted bands was measured, normalised to that of internal control, and expressed in intensity units/mm2. Values given are mean of 5 experiments ± SEM.
