Dependence of Proximal GC Boxes and Binding Transcription Factors in the Regulation of Basal and Valproic Acid-Induced Expression of t-PA

Figure 1

Schematic illustration of the proximal promoter region of the t-PA gene and the coverage of the insert used in presented reporter studies. Marked boxes illustrate previously described cis-acting elements [16, 17]; the cAMP responsive element- (CRE-) like site, the CAAT-binding transcription factor/nuclear factor-1- (CTF/NF1-) like binding site, and the three GC box elements (I, II, and III). Highlighted in black are elements that were subjected to site-directed mutagenesis and tested for involvement in induction by VPA. All positions in this study are given in relation to the major t-PA transcription initiation site (TIS, +1, positioned on chromosome 8:42,207,565) [18] and are according to the Dec. 2013 Genome Reference Consortium GRCh38 assembly obtained from the UCSC Genome Browser database (http://genome.ucsc.edu/).