Dependence of Proximal GC Boxes and Binding Transcription Factors in the Regulation of Basal and Valproic Acid-Induced Expression of t-PA

Figure 6

siRNA specificity in Sp2, Sp4, and KLF5 knocked HUVECs. mRNA expression of Sp2, Sp3, and Sp4 in HUVECs treated with siRNA targeting (a) Sp2 and (b) Sp4. (c) mRNA expression of KLF4, KLF5, and KLF6 in HUVECs treated with siRNA targeting KLF5. Mock = transfection reagent only; ASN = transfection with “All Star Negative” control siRNA. The results are presented as mean values ± SEM of three independent experiments. The statistical evaluation was performed using paired Student’s -test.

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