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International Journal of Zoology
Volume 2010, Article ID 482732, 5 pages
http://dx.doi.org/10.1155/2010/482732
Research Article

A Phylogenetic Index for Cichlid Microsatellite Primers

1Biology Department, Reed College, 3203 SE Woodstock Boulevard, Portland, OR 97202, USA
2Santa Catalina School, 1500 Mark Thomas Drive, Monterey, CA 93940, USA

Received 1 April 2010; Revised 28 September 2010; Accepted 12 October 2010

Academic Editor: Ajai Kumar Srivastav

Copyright © 2010 Robert D. Kunkle et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Microsatellites abound in most organisms and have proven useful for a range of genetic and genomic studies. Once primers have been created, they can be applied to populations or taxa that have diverged from the source taxon. We use PCR amplification, in a 96-well format, to determine the presence and absence of 46 microsatellite loci in 13 cichlid species. At least one primer set amplified a product in each species tested, and some products were present in nearly all species. These results are compared to the known phylogenetic relationships among cichlids. While we do not address intraspecies variation, our results present a phylogenetic index for the success of microsatellite PCR primer product amplification, thus providing information regarding a collection of primers that are applicable to wide range of species. Through the use of such a uniform primer panel, the potential impact for cross species would be increased.