Pathogens Associated with Sugarcane Borers, Diatraea spp. (Lepidoptera: Crambidae): A Review

Table 2

Entomopathogenic-fungi bioassays on Diatraea spp.

Host species	Pathogen	Bioassay	Result	Reference
D. saccharalis	B. bassiana	Immersing third instar larvae into a suspension of 10^8 conidia/mL	TL50 (conidia isolates Bb1 and 5): 4.3 days.; Mortality dry mycelium preparations: 21.3% (Bb1); 82.5% (Bb5) at 7 days after inoculation	Arcas et al., 1999 [26]
D. saccharalis	B. bassiana; Mycotrol strain GHA	Sprayed first, second and third instar larvae with 1 mL	LD50 in spores/mm^2; 1st instar: 72.1; 2nd instar: 384.3; 3rd instar: 777.0; Mean days of survival; 1st instar: 4.6; 2nd instar: 4.8; 3rd instar: 6.4	Legaspi et al., 2000 [27]
D. grandiosella	B. bassiana; Eight isolates	Dipped in conidial suspension (dosage not specified)	Mortality from 10 to 21%	Inglis et al., 2000 [5]
D. saccharalis	B. bassiana ATCC 20872 from Solenopsis invicta	Third instar larvae sprayed with suspension of 10^8 conida/mL	TL50 3.02 to 4.10 days	Marques et al., 2000 [28]
D. sacccharalis	B. bassiana ATCC 20872 from Solenopsis invicta	Third instar larvae sprayed with 3 mL of yeast-like cells or conidia	LC50 5.6 × 10^6 yeast/mL and; 4.8 × 10^6 conidia/mL	Alves et al., 2002 [29]
D. saccharalis	B. bassiana; IBCB from Brazil	1 cm larvae sprayed with 1 mL	CL50 1.58 × 10^7 conidia/mL at 6 days after inoculation	Wenzel et al., 2006 [30]