Studies on the Pinctada fucata BMP-2 Gene: Structural Similarity and Functional Conservation of Its Osteogenic Potential within the Animal Kingdom
Figure 7
Histochemical analysis. C3H10T1/2 cells were plated into 40 mm culture dishes for 2 days. Cells were then treated with 250 ng/mL PfBMP-2 synthetic polypeptide or 1 μg/mL synthetic polypeptide of the knuckle epitope of human BMP-2 for the indicated time periods. (a) Staining for alkaline phosphatase was performed after 21 days. (b) von Kossa staining at 21 days. 1: the PfBMP-2 synthetic polypeptide; 2: the synthetic polypeptide of the knuckle epitope of human BMP-2; 3: control culture. The white arrows indicate the areas of calcification. Cells were photographed at 100x magnification.
