Expression Pattern of Myogenic Regulatory Transcription Factor mRNAs in the Embryo and Adult Labeo rohita (Hamilton, 1822)

Table 1

Primers used for polymerase chain reaction amplification of Labeo MyoD, myf-5, myogenin, MEF2A, and 𝛽-actin.

Gene	Primer sequence		Annealing temperature	Source sequence for primer designing; (accession numbers are given)	Position of primers in source sequence
MyoD	Forward	5′-CGACTGAGCAAAGTCAACGA-3′	59.0°C	Ctenopharyngodon idella mRNA for MyoD; (GenBank accession: JQ793893.1)	296–315
	Reverse	5′-TTCCGTCTTCTCGACTGACA-3′			561–542
myf-5	Forward	5′-GCCAGGTCACTGTCTGCAAT-3′	58.5°C	Cyprinus  carpio mRNA for myf-5; (GenBank accession: AB012883.1)	202–221
	Reverse	5′-GCTCAGAGCTGCTTTCCATT-3′			479–460
Myogenin	Forward	5′-GGCTTCGACCAAACAGGATA-3′	59.0°C	Cyprinus  carpio mRNA for myogenin; (GenBank accession: AB012881.1)	232–251
	Reverse	5′-GCTCCTGGTGAGGAGACAAG-3′			376–357
MEF2A	Forward	5′-ACGGATCATGGATGAGAGGA-3′	58.8°C	Cyprinus  carpio mRNA for MEF2A; (GenBank accession: AB012884.1)	199–218
	Reverse	5′-TGACCGAAACAGTCATCTGG-3′			492–473
𝛽-Actin	Forward	5′-TGGAATCCTGTGGCATCCATGAAAC-3′	66.0°C	Li et al. 2004 [16]
	Reverse	5′-TAAAACGCAGCTCAGTAACAGTCCG-3′