Spermatogenic Waves and Expression of AR and ERs in Germ Cells of Podarcis sicula

Figure 1

Histology and mRNA distribution of AR and ERs in the testis during the mating period. (a, b, c) Mallory’s trichrome staining: (a) all stages of germ cells differentiation: spg, spcI, spcII, spd, and spz are evident in a seminiferous tubule; (b) spcI in preleptotene stage (arrows), the red nucleolus is still evident (magnification of (a)); (c) spcI in pachytene stage (arrows), note the condensation of chromatin to constitute chromosomes. The asterisk marks the round spermatids (magnification of (a)). (d, e, f) ISH with homologous probes: (d) expression of AR (the square indicates the spcI in pachytene stage); in (e) expression of ERα; (f) expression of ERβ; (g) ISH-negative control section by omitting the probes. The bar is 30 µm.

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