Table of Contents
Journal of Insects
Volume 2013, Article ID 504285, 5 pages
http://dx.doi.org/10.1155/2013/504285
Research Article

Phylogenetic Relationship of the Longhorn Grasshopper Ruspolia differens Serville (Orthoptera: Tettigoniidae) from Northwest Tanzania Based on 18S Ribosomal Nuclear Sequences

1Department of Life Sciences, Mkwawa University College of Education (UDSM), P.O. Box 2513, Iringa, Tanzania
2Department of Molecular Biology and Biotechnology, University of Dar es Salaam, Dar es Salaam, Tanzania

Received 16 March 2013; Revised 20 April 2013; Accepted 20 April 2013

Academic Editor: Francisco de Sousa Ramalho

Copyright © 2013 Nicodemus D. Matojo and Keneth M. Hosea. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Previously, the biology of the longhorn grasshopper Ruspolia differens Serville (Orthoptera: Tettigoniidae) from northwest Tanzania was mainly inferred based on the morphological and behavioural characters with which its taxonomic status was delineated. The present study complements the previous analysis by examining the phylogenetic relationship of this insect based on the nuclear ribosomal molecular evidence. In the approach, the 18S rDNA of this insect was extracted, amplified, sequenced, and aligned, and the resultant data were used to reconstruct and analyze the phylogeny of this insect based on the catalogued data.