Relative Toxicity of Leaf Extracts of Eucalyptus globulus and Centella asiatica against Mosquito Vectors Aedes aegypti and Anopheles stephensi
Table 1
Effects of relative toxicity of solvent extracts (hexane, diethyl ether, dichloromethane, and methanol) of the leaves of Eucalyptus globulus and Centella asiatica against the late third larval stage of BSV and JPN strains of Aedes aegypti and DLC and KNG strains of Anopheles stephensi.
Botanical name
Solvents
Species
Strain
LC50 (ppm) (UCL-LCL)
LC90 (ppm) (UCL-LCL)
Regression equation
df
Eucalyptus globulus
Hexane
Ae. aegypti
BSV
225.2 (210.9–263.5)
423.6 (400.4–598.9)
0.9364
0.1902
7
JPN
167.7 (152.1–186.7)
304 (266.3–400.9)
0.984
0.0238
6
An. stephensi
DLC
118.8 (88.1–151.2)
450.4 (373.8–953.3)
0.9137
0.1123
7
KNG
192.8 (154.7–255.1)
827 (655.2–1634)
0.9378
0.1134
7
Diethyl ether
Ae. aegypti
BSV
686.8 (630.8–756.4)
1216.7 (1073.7–1582.5)
0.9594
0.0244
7
JPN
318.8 (146.7–641.2)
2069.9 (1085.1–34888.5)
0.9114
0.1811
6
An. stephensi
DLC
464.1 (428.8–524.2)
800.1 (726.1–1050.7)
0.9382
0.0767
6
KNG
318 (270.6–391.7)
951.9 (737.4–1618.7)
0.9279
0.0605
6
Dichloromethane
Ae. aegypti
BSV
314.6 (276.1–385)
829.9 (677.1–1290.7)
0.9846
0.0832
6
JPN
362.2 (171.6–620.1)
2904.6 (1068.6–13925.1)
0.889
0.1362
6
An. stephensi
DLC
300.3 (256.3–394.2)
1235.4 (872.5–2283.9)
0.9747
0.1345
7
KNG
162 (112.5–199.4)
582.4 (452.6–1323.4)
0.9623
0.0536
7
Methanol
Ae. aegypti
BSV
1839.3 (1695.8–2049.7)
3235.9 (2930.2–4051.1)
0.9805
0.0504
7
JPN
1467.9 (1281–1786.8)
3530.2 (2911.7–5612.3)
0.9644
0.0634
5
An. stephensi
DLC
1263.3 (798.1–1817.3)
3614.3 (2988.3–12736.6)
0.8769
0.2807
7
KNG
889.6 (764.7–1124.3)
2655.1 (2177.0–4907.4)
0.9204
0.1292
6
Centella asiatica
Hexane
Ae. aegypti
BSV
246.5 (213.1–307.1)
593.1 (527.3–1013.9)
0.9284
0.1366
5
JPN
268.7 (174.4–374.7)
1606.8 (1082.1–5866.2)
0.929
0.0583
5
An. stephensi
DLC
50.6 (18.3–105)
328.8 (198.5–8730)
0.8489
0.2479
6
KNG
243.5 (221.1–274.2)
428.6 (362.7–584.5)
0.9963
0.0055
5
Diethyl Ether
Ae. aegypti
BSV
339.6 (258.1–472.3)
1599.3 (1173.6–3840.5)
0.8489
0.0844
5
JPN
134.5 (20.6–204.1)
770.8 (576.7–3191.7)
0.9362
0.0463
5
An. stephensi
DLC
241 (203.4–301.5)
630.7 (540.4–1116.2)
0.8489
0.1046
5
KNG
14.7 (3–35)
118 (63.7–17122)
0.8862
0.1491
5
Dichloromethane
Ae. aegypti
BSV
339.7 (28.1–746.3)
2127.7 (1241.9–403246)
0.9384
0.1913
5
JPN
837.6 (777.9–1030.2)
1627.2 (1542.3–2558.8)
0.9
0.1722
5
An. stephensi
DLC
236.8 (145.5–393.3)
3347.1 (1576.7–27927.2)
0.9338
0.0533
5
KNG
155.5 (20.9–295.5)
3022.9 (1732.5–33559.1)
0.8881
0.0528
5
Methanol
Ae. aegypti
BSV
776.5 (590.99–1090.78)
3853.4 (2728.01–10164.21)
0.8905
0.0966
5
JPN
721.5 (369.54–1358.04)
2806 (1866.07–32489.71)
0.8905
0.1928
5
An. stephensi
DLC
476.8 (461.94–493.49)
587.9 (567.22–643.99)
0.9832
0.0333
8
KNG
177 (119.28–225.09)
559.9 (483.97–1260.17)
0.9002
0.1107
5
LC50: lethal concentration that kills 50% of the exposed larvae, LC90: lethal concentration that kills 90% of the exposed larvae, UCL: upper confidence limit, LCL: lower confidence limit, : correlation coefficient, : Chi-square significant at < 0.05 level, and df: degree of freedom.
