Table 1: Effects of relative toxicity of solvent extracts (hexane, diethyl ether, dichloromethane, and methanol) of the leaves of Eucalyptus globulus and Centella asiatica against the late third larval stage of BSV and JPN strains of Aedes aegypti and DLC and KNG strains of Anopheles stephensi.

Botanical nameSolventsSpeciesStrainLC50 (ppm) (UCL-LCL)LC90 (ppm) (UCL-LCL)Regression equationdf

Eucalyptus  globulus HexaneAe. aegypti BSV225.2 (210.9–263.5)423.6 (400.4–598.9)0.93640.19027
JPN167.7 (152.1–186.7)304 (266.3–400.9)0.9840.02386
An. stephensi DLC118.8 (88.1–151.2)450.4 (373.8–953.3)0.91370.11237
KNG192.8 (154.7–255.1)827 (655.2–1634)0.93780.11347
Diethyl etherAe. aegypti BSV686.8 (630.8–756.4)1216.7 (1073.7–1582.5)0.95940.02447
JPN318.8 (146.7–641.2)2069.9 (1085.1–34888.5)0.91140.18116
An. stephensi DLC464.1 (428.8–524.2)800.1 (726.1–1050.7)0.93820.07676
KNG318 (270.6–391.7)951.9 (737.4–1618.7)0.92790.06056
DichloromethaneAe. aegypti BSV314.6 (276.1–385)829.9 (677.1–1290.7)0.98460.08326
JPN362.2 (171.6–620.1)2904.6 (1068.6–13925.1)0.8890.13626
An. stephensi DLC300.3 (256.3–394.2)1235.4 (872.5–2283.9)0.97470.13457
KNG162 (112.5–199.4)582.4 (452.6–1323.4)0.96230.05367
MethanolAe. aegypti BSV1839.3 (1695.8–2049.7)3235.9 (2930.2–4051.1)0.98050.05047
JPN1467.9 (1281–1786.8)3530.2 (2911.7–5612.3)0.96440.06345
An. stephensi DLC1263.3 (798.1–1817.3)3614.3 (2988.3–12736.6)0.87690.28077
KNG889.6 (764.7–1124.3)2655.1 (2177.0–4907.4)0.92040.12926

Centella  asiatica HexaneAe. aegypti BSV246.5 (213.1–307.1)593.1 (527.3–1013.9)0.92840.13665
JPN268.7 (174.4–374.7)1606.8 (1082.1–5866.2)0.9290.05835
An. stephensi DLC50.6 (18.3–105)328.8 (198.5–8730)0.84890.24796
KNG243.5 (221.1–274.2)428.6 (362.7–584.5)0.99630.00555
Diethyl EtherAe. aegypti BSV339.6 (258.1–472.3)1599.3 (1173.6–3840.5)0.84890.08445
JPN134.5 (20.6–204.1)770.8 (576.7–3191.7)0.93620.04635
An. stephensi DLC241 (203.4–301.5)630.7 (540.4–1116.2)0.84890.10465
KNG14.7 (3–35)118 (63.7–17122)0.88620.14915
DichloromethaneAe. aegypti BSV339.7 (28.1–746.3)2127.7 (1241.9–403246)0.93840.19135
JPN837.6 (777.9–1030.2)1627.2 (1542.3–2558.8)0.90.17225
An. stephensi DLC236.8 (145.5–393.3)3347.1 (1576.7–27927.2)0.93380.05335
KNG155.5 (20.9–295.5)3022.9 (1732.5–33559.1)0.88810.05285
MethanolAe. aegypti BSV776.5 (590.99–1090.78)3853.4 (2728.01–10164.21)0.89050.09665
JPN721.5 (369.54–1358.04)2806 (1866.07–32489.71)0.89050.19285
An. stephensi DLC476.8 (461.94–493.49)587.9 (567.22–643.99)0.98320.03338
KNG177 (119.28–225.09)559.9 (483.97–1260.17)0.90020.11075

LC50: lethal concentration that kills 50% of the exposed larvae, LC90: lethal concentration that kills 90% of the exposed larvae, UCL: upper confidence limit, LCL: lower confidence limit, : correlation coefficient, : Chi-square significant at < 0.05 level, and df: degree of freedom.