Combining Microarray Technology and Molecular Epidemiology to Identify Genes Associated with Invasive Group B Streptococcus
Table 3
Open reading frames (ORF) present in invasive strains but absent at least in two out of three commensal tester group B Streptococcus strains by comparative genomic hybridization and their presence among 949 GBS isolates and their prevalence ratio between invasive (n = 386) and colonizing ( n = 563) isolates.
transcriptional regulator, Cro/CI family-related protein
sag0832
371 (39%)
1.5 (1.29–1.77)
fibrinogen binding protein
sag1130
367 (39%)
1.1 (0.90–1.25)
hypothetical protein
sag1140
(b)
(b)
hypothetical protein
sag1207
(b)
(b)
hypothetical protein
sag1781
(b)
(b)
primase-related protein
sag1968
87 (9%)
1.1 (0.71–1.61)
hypothetical protein
sag1969
907 (96%)
1.0 (0.97–1.03)
ribosomal protein L11 methyltransferase
sag1974
(b)
(b)
MutT/nudix family protein
sag1975
(b)
(b)
hypothetical protein
sag1976
290 (31%)
0.9 (0.73–1.08)
hypothetical protein
sag1994
289 (30%)
1.0 (0.82–1.22)
hypothetical protein
sag1999
(b)
(b)
hypothetical protein
sag2021
395 (42%)
1.2 (1.00–1.36)
cell wall surface anchor family protein
sag2026
224 (24%)
1.1 (0.88–1.40)
membrane protein, putative
sag2027
(b)
(b)
ABC transporter, ATP-binding protein
sag2028
(b)
(b)
hypothetical protein
sag2045
364 (38%)
1.1 (0.90–1.25)
DNA topology modulation protein FlaR, putative
sag2057
(b)
(b)
leucyl-tRNA synthetase
sag2060
427 (45%)
1.3 (1.13–1.50)
glycosyl transferase, family 8
sag2061
437 (46%)
1.2 (1.07–1.41)
glycosyl transferase, family 8
sag2088
(b)
(b)
hypothetical protein
sag2147
687 (72%)
1.0 (0.9–1.06)
lipoprotein, putative
gbs0474
270 (28%)
1.5 (1.21–1.80)
hypothetical protein
(a) Prevalence with confidence interval not overlapping 1 are considered statistical significant. (b) These ORFs were not screened using LOS mostly because of small sizes. (c) Exact corresponding gene in strain 2603VR was not found by blast search while it was classified as present in 2603 VR by CGH.
