The Human Vaginal Bacterial Biota and Bacterial Vaginosis
Figure 2
Comparison of vaginal
bacterial species detected by broad range 16S rRNA gene PCR using two different
forward primers and the same reverse primer in one sample. The pie charts show the percentages
of clones in each library corresponding to specific bacterial 16S rRNA gene
sequences obtained using broad range PCR followed by cloning and sequencing in
a vaginal sample from a subject diagnosed with bacterial vaginosis. Data obtained using the 338f (a) primer
shows a balanced representation of clones while the data obtained using the 27f
(b) primer is skewed toward Atopobium vaginae. Note the absence of Gardnerella vaginalis clones in the clone library created with the
27f primer. BVAB denotes bacterial
vaginosis associated bacterium.
