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Interdisciplinary Perspectives on Infectious Diseases
Volume 2012, Article ID 124879, 7 pages
http://dx.doi.org/10.1155/2012/124879
Research Article

Antibiotic Resistance of Escherichia coli Serotypes from Cochin Estuary

1Department of Marine Biology, Microbiology and Biochemistry, School of Marine Sciences, Cochin University of Science and Technology, Lakeside Campus, Cochin 682 016, India
2Mathematics & Sciences Division, Richland Community College, One College Park, Decatur, IL 62521, USA
3MacMurray College, 447 East College Avenue, Jacksonville, IL 62650, USA
4Environment Genomics Laboratory, Civil and Environmental Engineering, Michigan State University, East Lansing, MI 48824, USA

Received 30 May 2012; Revised 21 July 2012; Accepted 31 July 2012

Academic Editor: Mary E. Marquart

Copyright © 2012 Divya P. Sukumaran et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study aimed at detecting the prevalence of antibiotic-resistant serotypes of Escherichia coli in Cochin estuary, India. E. coli strains were isolated during the period January 2010–December 2011 from five different stations set at Cochin estuary. Water samples from five different stations in Cochin estuary were collected on a monthly basis for a period of two years. Isolates were serotyped, antibiogram-phenotyped for twelve antimicrobial agents, and genotyped by polymerase chain reaction for uid gene that codes for β-D-glucuronidase. These E. coli strains from Cochin estuary were tested against twelve antibiotics to determine the prevalence of multiple antibiotic resistance among them. The results revealed that more than 53.33% of the isolates were multiple antibiotic resistant. Thirteen isolates showed resistance to sulphonamides and two of them contained the sul 1 gene. Class 1 integrons were detected in two E. coli strains which were resistant to more than seven antibiotics. In the present study, O serotyping, antibiotic sensitivity, and polymerase chain reaction were employed with the purpose of establishing the present distribution of multiple antibiotic-resistant serotypes, associated with E. coli isolated from different parts of Cochin estuary.