Inhibited Production of iNOS by Murine J774 Macrophages Occurs via a phoP-Regulated Differential Expression of NFκB and AP-1
Figure 1
Wild type S. typhimurium 14028 suppresses macrophage nitrite ion production via the phoP regulon but is inhibited by IFN-γ. Histograms show nitrite ion concentrations measured in supernatants from macrophages cultured with 14028 wild type and phoP mutants and uninfected controls and with or without the addition of IFN-γ. *Significant increase (P < 0.05) in nitrite ion concentration in cell supernatants compared to uninfected controls at equivalent time points. Significant increase (P < 0.05) in nitrite ion concentrations recovered from macrophage supernatants cultured with 14028 phoP mutants compared to 14028 wild type is also shown. Each point (and standard deviations) is mean values calculated from triplicate cultures replicated on 5 separate occasions.
