Developments in Diagnosis and Antileishmanial Drugs

Table 2

Diagnostic methods for leishmaniasis and PKDL.

Diagnostic methods	Test name	Clinical specimen	Sensitivity	References
Parasitic detection methods	LD bodies	Lymph node, bone marrow, splenic, and slit aspirates	spleen (93–99%) than for bone marrow (53–86%) or lymph node (53–65%) aspirates	[59, 76]
	Culture (MCM)	Blood, lymph node, bone marrow, splenic, and slit aspirates	100% in bone marrow and 77.8–100% in peripheral blood	[77]
	Culture (TCM)	Blood, lymph node, bone marrow, splenic, and slit aspirates	37.5–100% in bone marrow and 0–100% in peripheral blood	[77]
Serological methods	DAT	Serum, urine	94.8%	[78–81]
	ELISA/immunoblotting soluble antigens	Serum, urine	100%	[82–84]
	Recombinant antigens (rK39, rK26, rHSP70)	Blood, serum, and antigens	100%	[85–88]
Molecular methods	PCR	Blood, serum, urine, lymph node, bone marrow,splenic, and slit aspirates	73.2%	[89, 90]
	PCR-ELISA	Blood, serum	83.9%	[90]
	Real-time PCR	Lymph node, bone marrow, splenic, and slit aspirates	90–100%	[75]