Efficacy of Essential Oils of Thymus vulgaris and Origanum vulgare on Echinococcus granulosus
Scanning electron microscopy of E. granulosus protoscoleces incubated in vitro with thymol and essential oils of T. vulgaris and O. vulgare. (a) Evaginated control protoscolex (6 days p.i., 600x). (b) Invaginated control protoscolex (36 days p.i., 950x). (c) Altered protoscolex after 6 days p.i with T. vulgaris. Note the loss of microtriches at rostellar region (650x). (d) Invaginated altered protoscolex incubated with O. vulgare during 6 days (850x). (e) Scolex region of an evaginated protoscolex after 6 days with thymol. The shedding of microtriches can be observed (1400x). (f) Evaginated protoscolex (T. vulgaris, 36 days p.i.). Note the shedding of microtriches at the scolex region, the presence of numerous blebs, and the contraction of the soma region (1000x). (g) Altered and contracted soma region (O. vulgare, 36 days p.i.). Note the rostellar disorganization and shedding of microtriches (850x). (h) Completely altered protoscoleces (thymol, 24 days p.i., 900x).