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Interdisciplinary Perspectives on Infectious Diseases
Volume 2017, Article ID 7279830, 14 pages
Research Article

Association between Virulence Factors and Extended Spectrum Beta-Lactamase Producing Klebsiella pneumoniae Compared to Nonproducing Isolates

1Microbiology & Immunology Department, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt
2Department of Pharmaceutical Sciences, College of Pharmacy, Princess Norah Bint Abdulrahman University, Riyadh 11671, Saudi Arabia

Correspondence should be addressed to Rasha Fathy Barwa; moc.liamg@0002ahsar

Received 12 January 2017; Revised 6 April 2017; Accepted 26 April 2017; Published 8 June 2017

Academic Editor: Mary E. Marquart

Copyright © 2017 Mustafa Muhammad Gharrah et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Klebsiella pneumoniae is considered an important opportunistic multidrug-resistant pathogen. Extended spectrum β-lactamases (ESBLs) and expression of a multitude of virulence factors may work in a harmony resulting in treatment failure. This study was undertaken to compare the virulence characteristics and genetic relatedness between ESBL and non-ESBL producing K. pneumoniae. Methods. Antibiotic sensitivity test of all isolates was determined by disc diffusion assay. Phenotypic and genotypic detection of ESBL were done. Various virulence factors and some virulence factor-associated genes were screened. Random amplified polymorphic DNA (RAPD) was employed to investigate the genetic fingerprints of ESBL from non-ESBL producing K. pneumoniae. Results. 50% of isolates were ESBL producers. A significant association was observed between ESBL production and biofilm (strong and moderate), serum resistance, and iss gene. Moreover, significant association between non-ESBL producers and hypermucoviscosity was identified. Dendogram analysis of RAPD profile classified K. pneumoniae isolates into four clusters (a, b, c, and d). Seventy-six percent of ESBL producers belonged to cluster a. In conclusion, this study suggests a correlation between ESBL production and some virulence factors. Therefore, success of treatment depends mainly on increased clinicians awareness and enhanced testing by laboratories to reduce the spread of these isolates.