A Novel Vaccine Using Nanoparticle Platform to Present Immunogenic M2e against Avian Influenza Infection

Table 2

Overview of immunization regimen.

Group	Dose (μg)	Inoculum	Adjuvant	Vaccination	Challenge
G1	75	Mono-M2e	−	+	+
G2	75	Mono-M2e	+	+	+
G3	75	Tetra-M2e	−	+	+
G4	75	Tetra-M2e	+	+	+
G5	—	PBS	−	−	+
G6	—	Nonvaccinated	−	−	−

Complete Freund’s adjuvant was used for the priming vaccine followed by incomplete Freund’s adjuvant for boosters. Challenge with LPAI subtype H5N2 was performed at 8 weeks of age using oculonasal route.