Table of Contents
Influenza Research and Treatment
Volume 2013, Article ID 656825, 8 pages
Research Article

Detection and Isolation of Airborne Influenza A H3N2 Virus Using a Sioutas Personal Cascade Impactor Sampler

1Department of Environmental and Global Health, College of Public Health and Health Professions, P.O. Box 100188, Gainesville, FL 32610, USA
2Emerging Pathogens Institute, University of Florida, Gainesville, FL 32610, USA

Received 27 May 2013; Revised 3 September 2013; Accepted 13 September 2013

Academic Editor: Ian Barr

Copyright © 2013 John A. Lednicky and Julia C. Loeb. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The air we breathe contains microorganisms that can cause infectious respiratory diseases. After two occupants of an apartment were diagnosed with influenza in February of 2013, efforts were made to detect and isolate airborne influenza virus using two different types of active air samplers: a Sioutas Personal Cascade Impactor Sampler (PCIS) and an SKC BioSampler. The PCIS collects size-fractionated particles by impaction on polytetrafluoroethylene filters, whereas the SKC BioSampler collects airborne particles in liquid media. Influenza H3N2 virus was collected by both types of air samplers. The PCIS collected a range of particle sizes containing influenza virus near one of the sick individuals but only ultrafine particles when the samplers were positioned farther away. Viable virus was present in the liquid collection media of the SKC BioSampler and some PCIS filters. These findings suggest that influenza patients produce ultrafine aerosol particles that contain viable virus.