Table of Contents
Influenza Research and Treatment
Volume 2014 (2014), Article ID 547839, 6 pages
Research Article

Effect of the PB2 and M Genes on the Replication of H6 Influenza Virus in Chickens

1Laboratory of Veterinary Microbiology, Faculty of Agriculture, Tottori University, 4-101 Koyama, Tottori 680-8553, Japan
2Centre for Influenza Research, School of Public Health, The University of Hong Kong, Hong Kong
3Division of Virology, Department of Infectious Diseases, St. Jude Children’s Research Hospital, Memphis, TN 38105, USA
4Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo 001-0020, Japan

Received 14 November 2013; Revised 27 December 2013; Accepted 6 January 2014; Published 18 February 2014

Academic Editor: Daniel R. Perez

Copyright © 2014 Hiroichi Ozaki et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


H6 subtype influenza viruses are commonly isolated from wild aquatic birds. However, limited information is available regarding H6 influenza virus isolated from chickens. We compared the viral genome segment between A/chicken/Hong Kong/W312/97 (H6N1), which was able to grow in chicken trachea, and A/duck/Shantou/5540/01 (H6N2), which was isolated from wild aquatic duck, to explore the factors for effective replication in chicken. When chickens were inoculated with reassortants (W312 background), the replication of viruses with PB2 and M genes derived from the duck strain was significantly reduced. Chimeras of PB2 and M proteins, encoding the C-terminal region of the PB2 protein and the M2 protein from W312, were required for efficient replication in canine-derived (MDCK) cells and in chicken trachea. These results indicate that host range may be determined by some types of internal proteins such as PB2 and M2, as well as by surface glycoprotein like hemagglutinin.