Table of Contents
ISRN Ophthalmology
Volume 2011, Article ID 184295, 6 pages
Research Article

Ginsenoside-Rb1 Induces ARPE-19 Proliferation and Reduces VEGF Release

Department of Biology, The University of Texas at San Antonio, San Antonio, TX 78249, USA

Received 18 October 2011; Accepted 10 November 2011

Academic Editors: A. Barak and H. Tomita

Copyright © 2011 Brandi S. Betts et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Rb1, a ginsenoside from ginseng root extract, possesses antiangiogenic effects, but its role on ocular cells has not been studied. We hypothesize that Rb1 inhibits the production of the angiogenic cytokine VEGF from ARPE-19 cells, leading to a significant reduction in the proliferation of ocular vasculatures. Data from our experiments show that Rb1 induced an increase in the number of ARPE cells in culture, while VEGF release (pg/10,000 viable cells) was significantly reduced. Treatment with VEGF and cotreatment with Rb1 and VEGF showed that this Rb1-induced cell proliferation was mediated by VEGF. Because VEGF from RPE plays a major role in promoting angiogenesis in ocular vasculatures. Our finding that Rb1 inhibits the release of VEGF from RPE cells suggests that Rb1 has a significant role in the eye to protect against angiogenic diseases such as age-related macular degeneration.