Table of Contents
ISRN Immunology
Volume 2011, Article ID 186541, 11 pages
Research Article

Immunosuppressive Factor MNSFβ Regulates Cytokine Secretion by Mouse Lymphocytes and Is Involved in Interactions between the Mouse Embryo and Endometrial Cells In Vitro

1Department of Immunology, School of Basic Medical Sciences, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
2NPFPC Key Laboratory of Contraceptive Drugs and Devices, Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China
3Prince Henry's Institute of Medical Research, Clayton, VIC 3168, Australia

Received 5 August 2011; Accepted 5 September 2011

Academic Editors: E. K. Kapsogeorgou and G. Marone

Copyright © 2011 Yaping He et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Identification of isolated primary mouse endometrial cell types by immunofluorescent staining. The endometrial cells were cultured on coverslips, fixed in 4% paraformaldehyde for 10 minutes at room temperature, and washed three times with PBS. To block the non-specific immune reaction, the cells were incubated in 10% goat serum in PBS for 30 min at room temperature followed by incubation with Row KRT-18 polyclonal antibody (the identification of endometrial epithelial cell-specific anti-keratin 18 expression) (1 : 100) and Row Vimentin polyclonal antibody (detection of endometrial stromal cell specific marker vimentin) respectively in blocking solution at 37°C for 1 hours. Cells were then washed in PBS three times, and incubated with Alexa Fluor 488-labeled goat anti-rabbit IgG (H+L) antibody (Invitrogen, USA) at a dilution ratio of 1 : 5000 in 10% goat serum in PBS at 37°C for 1 hour. After washing, the results were observed and recorded by fluorescence microscopy (Nikon TE2000-U, Japan).

  1. Supplementary Material