Table of Contents
ISRN Pathology
Volume 2011, Article ID 219790, 9 pages
Research Article

Evaluation of the In Situ Hybridization Signal Patterns of Liquid-Based Cytological Human Papillomavirus Specimens for Diagnosing Squamous Intraepithelial Lesion

1Department of Clinical Laboratory, National Hospital Organization Hamada Medical Center, Hamada 697-8511, Japan
2Department of Obstetrics and Gynecology, National Hospital Organization Hamada Medical Center, Hamada 697-8511, Japan
3Department of Obstetrics and Gynecology, Tokuyama Central Hospital, Shunan 745-8522, Japan
4Department of Functional Pathology, Shimane University School of Medicine, Izumo 693-8511, Japan
5Institute for Clinical Research, National Hospital Organization, Kure Medical Center and Chugoku Cancer Center, Kure 737-0023, Japan

Received 15 April 2011; Accepted 8 June 2011

Academic Editors: A.-J. Kruse, C. K. Panda, and R. S. Saad

Copyright ยฉ 2011 Katsunari Ishida et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


To examine the diagnostic utility for squamous intraepithelial lesion (SIL) by cytological in situ hybridization (c-ISH) for the human papillomavirus using liquid-based cytology specimens, we investigated c-ISH signal patterns in the cases of low-grade SIL (LSIL), atypical squamous cells of undetermined significance (ASC-US), and high-grade SIL (HSIL). Episomal (E) and/or integrated (I) signals were observed. Two signal patterns ( ๐ธ โ‰ง ๐ผ or ๐ผ > ๐ธ ) were obtained by counting the number of ๐ธ + or ๐ผ + cells. ๐ธ โ‰ง ๐ผ was specific to LSIL and ASC-US (10/12); ๐ผ > ๐ธ , to HSIL (9/11) ( ๐‘ƒ < 0 . 0 1 , χ2 test), suggesting significant utility of c-ISH in diagnosing SIL. In the cell fraction, ๐ธ โ‰ง ๐ผ in large cells was dominant in LSIL. Two cases of ๐ผ > ๐ธ in large cells of LSIL showed HPV persistence and/or progression during follow-up. Thus, c-ISH is useful in routine testing for diagnosing cervical dysplastic lesions, especially for detecting LSIL suspected for progression.