Evaluation of the In Situ Hybridization Signal Patterns of Liquid-Based Cytological Human Papillomavirus Specimens for Diagnosing Squamous Intraepithelial Lesion

Table 3

Combination of Papanicolaou and c-ISH signal patterns to detect CIN1 or CIN2/3.

		histology
		metaplasia/cervicitis	CIN1	CIN2/3		LSIL-CIN1	HSIL-CIN2/3
(1) cytology:	NILM	3	0	0	sensitivity	69.2% (9/13)	76.9% (10/13)
Pap	LSIL	1	9	3	specificity	80.0% (16/20)	90.0% (18/20)
	ASC-US	3	2	0	PPV	69.2% (9/13)	83.3% (10/12)
	HSIL	0	2	10	NPV	80.0% (16/20)	85.7% (18/21)
(2) cytology:	NILM	0	0	0	sensitivity	88.9% (8/9)	66.7% (2/3)
Pap with c-ISH	LSIL	0	8	1	specificity	66.7% (2/3)	100% (9/9)
(E ≧ I)	ASC-US	0	1	0	PPV	88.9% (8/9)	100% (2/2)
	HSIL	0	0	2	NPV	66.7% (2/3)	90.0% (9/10)
(3) cytology:	NILM	0	0	0	sensitivity	50% (1/2)	88.9% (8/9)
Pap with c-ISH	LSIL	0	1	1	specificity	88.9% (8/9)	50.0% (1/2)
(I > E)	ASC-US	0	0	0	PPV	50% (1/2)	88.9% (8/9)
	HSIL	0	1	8	NPV	88.9% (8/9)	50.0% (1/2)

Pap, Papacicolaou; NILM, negative for intraepithelial lesion; LSIL, low grade squamous intraepithelial lesion;
ASC-US, atypical squamous cells of undetermined significance; HSIL, high grade squamous intraepithelial lesion;
CIN, cervical intraepithelial neoplasia; PPV, positive predictive value; NPV, negative predictive value
The calculated sensitivity, d/b+d; specificity, a/a+c; PPV, d/c+d; NPV, a/a+b.
LISL-CIN1: aCytology was NILM, ASC-US, HSIL and histology was metaplasia/cervicitis, CIN2/3 bCytology was NILM, ASC-US, HSIL and histology was CIN1 cCytology was LSIL and histology was metaplasia/cervicitis, CIN2/3 dCytology was LSIL and histology was CIN1. HSIL-CIN2/3; aCytology was NILM, ASC-US, LSIL and histology was metaplasia/cervicitis, CIN1 bCytology was NILM, ASC-US, LSIL and histology was CIN2/3 cCytology was HSIL and histology was metaplasia/cervicitis, CIN1 dCytology was HSIL and histology was CIN2/3.