Identification of Potential Drug Targets Implicated in Parkinson's Disease from Human Genome: Insights of Using Fused Domains in Hypothetical Proteins as Probes

Table 5

Mutational analysis of the hypothetical proteins with ubiquitin domains.

Sl. no.	Gi ID	Mutational positions	Function as predicted by protein mutant database (PMD)
1	5912028	Nil	No change
2	37589137	I30V	Stability is retained.
		G35K	Melting temperature at pH 3.0 decreases.
3	44662819	I30V	Stability is retained.
		G35K	Melting temperature at pH 3.0 decreases.
4	57997480	G35S	Melting temperature at pH 3.0 decreases.
		K48R	Increase in morphologic response of cells to canavanine, accumulation of high-molecular-weight ubiquitin conjugates and proteome substrates is observed.
5	12052812	R42L	Ubiquitin adenylate affinity for E1 protein decreases.
		G35K	Melting temperature at pH 3.0 decreases.
6	10241759	R42L	Ubiquitin adenylate affinity for E1 protein decreases.
		G35K	Melting temperature at pH 3.0 decreases.