Table of Contents
ISRN Pathology
Volume 2011, Article ID 275102, 12 pages
http://dx.doi.org/10.5402/2011/275102
Research Article

Investigation of Archived Formalin-Fixed Paraffin-Embedded Pancreatic Tissue with Whole-Genome Gene Expression Microarray

1Department of Clinical Genetics, Odense University Hospital, Sdr Boulevard 29, 5000 Odense C, Denmark
2Great Ormond Street Children’s Hospital NHS Trust and Institute of Child Health, London WC1N 1EH, UK
3H.C. Andersen Children’s Hospital, Odense University Hospital, Sdr Boulevard 29, 5000 Odense C, Denmark

Received 9 August 2011; Accepted 26 September 2011

Academic Editors: A. B. Galosi and T. Yazawa

Copyright © 2011 Nete V. Michelsen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The use of formalin-fixed, paraffin-embedded (FFPE) tissue overcomes the most prominent issues related to research on relatively rare diseases: limited sample size, availability of control tissue, and time frame. The use of FFPE pancreatic tissue in GEM may be especially challenging due to its very high amounts of ribonucleases compared to other tissues/organs. In choosing pancreatic tissue, we therefore indirectly address the applicability of other FFPE tissues to gene expression microarray (GEM). GEM was performed on archived, routinely fixed, FFPE pancreatic tissue from patients with congenital hyperinsulinism (CHI), insulinoma, and deceased age-appropriate neonates, using whole-genome arrays. Although ribonuclease-rich, we obtained biologically relevant and disease-specific, significant genes; cancer-related genes; genes involved in (a) the regulation of insulin secretion and synthesis, (b) amino acid metabolism, and (c) calcium ion homeostasis. These results should encourage future research and GEM studies on FFPE tissue from the invaluable biobanks available at the departments of pathology worldwide.