Mgat5 Deficiency in T Cells and Experimental Autoimmune Encephalomyelitis

Figure 2

Mice deficient in 𝑀𝑔𝑎𝑡5 are more susceptible to EAE. (a–e), Splenocytes were isolated from 𝑀𝑔𝑎𝑡5^−/− mice 10 days after immunization with MBP + complete Freund’s adjuvant (CFA) and restimulated in vitro with MBP for two days. 2.7 million CD3⁺ T cells were injected into naïve 𝑀𝑔𝑎𝑡5^+/+ or 𝑀𝑔𝑎𝑡5^−/− mice and scored for clinical signs of EAE daily for 40 days. Mice were weighed daily throughout the duration of the experiment and used to determine maximum weight fluctuations. P value for EAE incidence was determined by Fisher’s exact test. 𝑃 values for EAE mean clinical score, disease duration, and the highest clinical score were determined by the Mann-Whitney test.

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