Table of Contents
ISRN Materials Science
Volume 2011, Article ID 389872, 11 pages
Review Article

Degradable PHBHHx Modified by the Silk Fibroin for the Applications of Cardiovascular Tissue Engineering

The Key Laboratory of Molecular Engineering of Polymers, Ministry of Education, Department of Macromolecular Science, Fudan University, Shanghai 200433, China

Received 19 August 2011; Accepted 18 September 2011

Academic Editor: J. Y. Lu

Copyright © 2011 Hua-Xiao Yang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Biodegradable polymer, poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx), has been used to fabricate the tissue-engineered cardiovascular scaffolds due to its controllable and suitable mechanical properties. However, the unsound biocompatibility of PHBHHx is still the unsolved problem till now. Silk fibroin (SF) with low inflammation, no blood clotting, and good cell and tissue compatibility in vitro and vivo is adopted as a surface modificator to improve the biocompatibility of PHBHHx. The adhesion of SF on PHBHHx surface was investigated by infrared spectroscopy and water contact angle. The proliferation and morphologies of human fibroblasts, human smooth muscle cells, endothelial-like cell line ECV304, and umbilical vein endothelial cells on the SF-modified PHBHHx were studied by the assays of MTT, H&E staining, DAPI staining, and the scanning electron microscopy. In sum, SF-modified PHBHHx scaffolds are highly biocompatible with cardiovascular-related cells, demonstrating its potential help for the extensive applications of PHBHHx in the cardiovascular regeneration.