Figure 3: Reverse transcription polymerase chain reaction (RT-PCR) expression analysis. 2% agarose gel electrophoresis of PCR products based on 100 ng of total RNA isolated from rat forebrain. cDNA was used to check for the amplification of a 409 bp fragment of the mammalian target of rapamycin (mTOR) transcript. Cyclophilin A (351 bp fragment) was used as a reference gene. Lane 1: sample from xenon-treated animal and lane 2: sample from control animal.